Domestication cultivation method of dendrobium candidum tissue culture seedling

A technology of Dendrobium officinale and a cultivation method, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as inconvenience in planting management and promotion, difficulty in collecting Dendrobium officinale seeds, and expanding planting area, etc., so as to improve the survival rate of transplanting , Quickly adapt to the natural environment, improve the effect of absorption function

Inactive Publication Date: 2013-09-25
重庆市秀山红星中药材开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because the seeds of Dendrobium candidum are difficult to collect, the germination rate of the seeds is low, and the seed propagation takes a long time to raise seedlings, which greatly increases the cultivation cost of Dendrobium candidum, which is not conducive to the artificial large-scale planting of Dendrobium candidum
Therefore, in the current artificial cultivation, propagation mainly relies on division propagation and cutting propagation, but this method has a low propagation coefficient, is not economical, and limits the yield potential of Dendrobium candidum, which is inconvenient for planting management and promotion, so the expansion of planting area is restrained
People have started artificial propagation, and many places have adopted the method of tissue culture propagation to obtain a large number of seedlings quickly; because tissue culture propagation is carried out in an artificial environment, its self-supporting ability is very poor, especially the absorption function of its roots, stems, etc. The dredging and transportation function of the leaves and the photosynthetic function of the leaves cannot adapt to the external environment, and they cannot fully perform their functions, which leads to a very low survival rate of transplanting. Therefore, domestication is required to successfully transplant to improve its survival. Rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The sawdust was directly exposed to the sun until the water content was 4%, and then soaked with 1000 times thiophanate-methyl + 800 times dichlorvos for 12 hours to make a tissue culture substrate for domesticated seedlings.

[0019] On March 20, 2012, the test team of our company moved the tissue culture seedlings together with 100 bottles of seedling bottles to the seedling hardening room for seedling hardening, and received natural light through the sunshade net. The intensity of natural light was controlled between 2000lx-3000lx in the first week In the second week, control the intensity of natural light between 3000lx-4000lx, in the third week, control the intensity of natural light between 4000lx-5000lx, and control the temperature in the seedling room between 18°C-28°C. The relative humidity is controlled between 50%-60%; on April 10, open the bottle cap, and let the tissue culture seedlings directly contact with the outdoor environment for 2 days; Tissue cultur...

Embodiment 2

[0021] The sawdust is directly exposed to the sun until the water content is 4.5%, and then soaked with 1000 times of thiophanate-methyl + 800 times of dichlorvos for 12 hours to make a tissue culture substrate for domesticated seedlings.

[0022]On April 10, 2012, the test team of our company moved the tissue cultured seedlings together with 150 bottles of seedling bottles to the seedling hardening room for hardening, and received natural light through the sunshade net. In the first week, the intensity of natural light was controlled between 2000lx-3000lx In the second week, control the intensity of natural light between 3000lx-4000lx, in the third week, control the intensity of natural light between 4000lx-5000lx, and control the temperature in the seedling room between 18°C-28°C. The relative humidity is controlled between 50%-60%; on May 1, open the bottle cap, let the tissue culture seedlings directly contact the outdoor environment for 3 days, and then select the seedling...

Embodiment 3

[0024] The sawdust was directly exposed to the sun until the water content was 5%, and then soaked with thiophanate-methyl 1000 times + dichlorvos 800 times for 12 hours to make a tissue culture substrate for domesticated seedlings.

[0025] On April 20, 2012, the test team of our company moved the tissue cultured seedlings together with 100 bottles of seedlings to the seedling hardening room for hardening, and received natural light through the sunshade net, and controlled the intensity of natural light between 2000lx-3000lx in the first week In the second week, control the intensity of natural light between 3000lx-4000lx, in the third week, control the intensity of natural light between 4000lx-5000lx, and control the temperature in the seedling room between 18°C-28°C. The relative humidity is controlled between 50%-60%; on May 12, open the bottle cap, let the tissue culture seedlings directly contact the outdoor environment for 3 days, and then select the seedlings with a len...

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Abstract

The invention relates to a domestication cultivation method of a dendrobium candidum tissue culture seedling and belongs to the technical field of planting of traditional Chinese medicinal materials. The domestication cultivation method of the dendrobium candidum tissue culture seedling comprises the following steps of: preparing a matrix, exercising seedling, taking seedlings out bottles, carrying out domestication cultivation, and carrying out domestication management. The domestication cultivation method of the dendrobium candidum tissue culture seedling has the beneficial effects that an appropriate transition method from experiment bottles to nature is provided for the dendrobium candidum tissue culture seedling, the tissue culture seedling can rapidly improve an absorption function of root hairs, a dredging transportation function of stems and a photosynthesis function of blades and can adapt to the natural environment more rapidly, the transplanting survival rate of the tissue culture seedling can be improved, and domestication seedling transplanting survival rate can be more than 95% by applying the domestication cultivation method of the dendrobium candidum tissue culture seedling.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, in particular to a method for domesticating and cultivating dendrobium officinale tissue culture seedlings. Background technique [0002] Dendrobium is a traditional precious Chinese herbal medicine in my country, which is recorded in all previous editions of Chinese Pharmacopoeia. The original plant of the traditional Chinese medicine Dendrobium is Dendrobium (Orchidaceae) ( Dendrobium Sw.) Herbaceous perennials. Dendrobium is one of the largest genera of Orchidaceae. There are more than 1,000 species in the world. There are 74 species and 2 varieties of Dendrobium plants in my country, of which more than 50 species are in commercial circulation. Among them, Dendrobium candidum is the most precious and the best of all Dendrobium. Dendrobium officinale is an endangered species, a national second-class key protected plant, and a rare and rare Chinese medicinal mate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨再江陈荣万联新袁志强邹代学
Owner 重庆市秀山红星中药材开发有限公司
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