Grifola frondosus solid state fermentation functional beverage and its preparation method
A technology of solid-state fermentation and grifola frondosa, which is applied in food preparation, application, food science, etc., can solve problems such as few and unreported research, and achieve the effect of enriching nutritional value
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[0035] The preparation method of the solid-state fermented functional drink of Grifola frondosa comprises the following steps:
[0036] A, use the shake flask culture medium that contains shiitake mushroom or shiitake mushroom stipe and apricot leaf, make mycosphere through shaking flask culture, make protoplast after enzymatic hydrolysis;
[0037] A-1 After activating the purchased Grifola frondosa strains, carry out shake flask culture, make the protoplasts after enzymatic hydrolysis of the obtained bacterium balls, and regenerate them after ultraviolet mutagenesis treatment,
[0038] The shake flask culture medium composition that adopts is: Glucose 20g, potato 120g, bran 50g, shiitake mushroom or mushroom stalk 22g, apricot leaf 8g, add distilled water and dilute to 1000mL;
[0039] Potatoes and wheat bran are boiled for 20 minutes, filtered, and the filtrate is obtained; shiitake mushrooms or shiitake stalks, and apricot leaves are dried, crushed, and sieved through an 80...
Embodiment 2
[0095] In the embodiment of the present invention, the solid-state fermentation compound of Grifola frondosa-lentinus edodes-hawthorn leaves, and then compatible components of mountain apricot, Codonopsis pilosula, wolfberry, Panax notoginseng, Luo Han Guo, the ratio is 5:1:1:1:0.1:0.1 ( w / w); change Panax notoginseng to kudzu root, the same as the rest.
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