Method for detecting sulfide ions

A sulfide ion and linear regression equation technology, applied in the field of sulfide ion detection, can solve the problems of cumbersome detection process and achieve the effect of simple detection means, high sensitivity and selectivity, and low cost

Inactive Publication Date: 2013-09-25
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the above methods involve the synthesis of detection reagents, or the detection process is cumbersome

Method used

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  • Method for detecting sulfide ions
  • Method for detecting sulfide ions
  • Method for detecting sulfide ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Prepare a HEPES buffer solution with a pH of 7.0 and a concentration of 10 mM, and prepare a 2 mM perylene-3,4,9,10-tetracarboxylic dianhydride solution with DMSO; mix 2 mL of HEPES buffer solution and 10 μL of perylene-3,4, Add 9,10-tetracarboxylic dianhydride DMSO solution to a clean fluorescence cuvette, take S 2– The solution is gradually added to the cuvette with a micro-injector, and is detected on the fluorescence spectrophotometer while adding the sample. 2– The addition of , the fluorescence intensity at 499nm gradually increased. Fluorescence emission map see figure 1 .

Embodiment 2

[0021] Prepare a HEPES buffer solution with a pH of 7.0 and a concentration of 10 mM, and prepare a 2 mM perylene-3,4,9,10-tetracarboxylic dianhydride solution with DMSO; add 2 mL of HEPES buffer to each of the 15 fluorescent cuvettes solution and 10 μL perylene-3,4,9,10-tetracarboxylic dianhydride DMSO solution, and then add 20 molar equivalents of S 2– , and 100 molar equivalents of various other analytes: Cys, Hcy, GSH, CN – ,SCN – , HSO 3 – , HCO 3 – , SO 4 2– ,N 3 – ,P 2 o 7 4– , citrate, HPO 4 2– ,F – , detected on a fluorescence spectrophotometer, draw the histogram of the 499nm fluorescence intensity corresponding to different analytes, and obtain the fluorescence emission diagram (see figure 2 ). S 2– The fluorescence intensity of perylene-3,4,9,10-tetracarboxylic dianhydride changed from 35 to about 830, and other analytes basically did not cause the fluorescence intensity of perylene-3,4,9,10-tetracarboxylic dianhydride The change.

[0022] It ha...

Embodiment 3

[0024] Prepare a HEPES buffer solution with a pH of 7.0 and a concentration of 10 mM, prepare a 2 mM perylene-3,4,9,10-tetracarboxylic dianhydride solution with DMSO, and prepare a 2 mM S 2– solution; add 2mL of HEPES buffer solution and 10μL of perylene-3,4,9,10-tetracarboxylic dianhydride DMSO solution to the fluorescence cuvette, add S 2– When the volume of the solution is 10, 20, 40, 60, 80, 100, 120, 140, 160, 180, 200μL, the fluorescence intensity F corresponding to 499nm measured on a fluorescence spectrophotometer is 76, 119, 205, 281, 370 , 448, 517, 599, 677, 742, 828, with S 2- The concentration is the abscissa, and the relative fluorescence intensity F-F 0 Plot the graph for the ordinate, F 0 ﹦35, get S 2– Concentration working curve (see image 3 ); the linear regression equation is: F-F 0 =-2.302+4.336c, the unit of c is 10 -6 mol / L.

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Abstract

The invention provides a fluorescence detection method for sulfide ions, which is a method for quantitatively detecting the sulfide ions based on perylene-3, 4, 9, 10-tetracarboxylic acid dianhydride. Specifically, the method comprises the following steps of: adding sulfide ions into HEPES (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid) containing the perylene-3, 4, 9, 10-tetracarboxylic acid dianhydride with the pH of 7.0 (without fluorescence emission), producing strong fluorescence emission and realizing the detection of the sulfide ions. According to the detection method, high sensitivity and selectivity are showed against the sulfide ions, and the method has the advantages of low price of a detection reagent, simplicity and convenience in detection process, sensitivity, quickness and accurate detection result.

Description

technical field [0001] The invention relates to a sulfide ion detection technology, in particular to a method for rapid and quantitative fluorescence detection of sulfide ions using perylene-3,4,9,10-tetracarboxylic dianhydride as a fluorescent reagent. Background technique [0002] Hydrogen sulfide (H 2 S), which has an unpleasant smell of rotten eggs and is traditionally considered a poisonous gas. However, recent studies have shown that hydrogen sulfide is an endogenous gas signaling compound (gas transporter) that is as important as two other known endogenous gas signaling molecules: nitric oxide (NO) and carbon monoxide ( CO) on a par. In recent years, however, evidence has emerged that, in mammals, this small molecule can act as a cell signal transducer. Endogenous and exogenous H 2 S production has been shown to be protective in many pathologies. For example, H 2 S relaxes vascular smooth muscle, causes isolated blood vessels to dilate, and lowers blood pressure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 阴彩霞杨瑜涛霍方俊
Owner SHANXI UNIV
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