Method for detecting DNA mutation based on graphene sensor

A graphene and sensor technology, applied in the field of biology, can solve problems such as sensitivity and specificity limitations, and achieve the effect of high specificity and good stability

Inactive Publication Date: 2013-09-25
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of these existing methods still have certain limita

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  • Method for detecting DNA mutation based on graphene sensor
  • Method for detecting DNA mutation based on graphene sensor
  • Method for detecting DNA mutation based on graphene sensor

Examples

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Embodiment 1

[0017] Example 1: Detection of DNA methylation based on graphene sensors

[0018] For the entire testing process, see figure 1 , first prepare a graphene device with electrodes at both ends, S is the source, D is the drain, assemble the connecting molecules on the graphene surface of the graphene device, connect the DNA to the graphene surface, and perform related detection.

[0019] 1) Preparation of device: a) Use a flexible polymethyl methacrylate (PMMA) polymer film as a support, spin-coat PMMA film on the copper foil with grown graphene, bake at 180°C for 2min, and then place the sample Placed in a saturated ferric nitrate solution, the copper foil is etched away, and the graphene embedded in the PMMA film is separated. The PMMA film carrying the graphene film can be attached to the silicon substrate, and then the PMMA film can be removed with acetone.

[0020] b) Spin-coat photoresist on the graphene, denote as photoresist 1, expose photoresist 1 to obtain a mark patte...

Embodiment 2

[0027] Embodiment 2: Detect SNP based on graphene sensor

[0028] 1) Preparation of device: a) Use a flexible polymethyl methacrylate (PMMA) polymer film as a support, spin-coat PMMA film on the copper foil with grown graphene, bake at 180°C for 2min, and then place the sample Placed in a saturated ferric nitrate solution, the copper foil is etched away, and the graphene embedded in the PMMA film is separated. The PMMA film carrying the graphene film can be attached to the silicon substrate, and then the PMMA film can be removed with acetone.

[0029] b) Spin-coat photoresist on the graphene, denote as photoresist 1, expose photoresist 1 to obtain a mark pattern, and vapor-deposit a thickness of 1-100nm (such as 80nm) Cr layer, 20 -1000nm (as 200nm) Au layer, remove the photoresist 1, left the vapor-deposited metal mark at the graphene exposure position, and the metal mark is used for the position alignment mark of next two steps photolithography; Wherein, the The graphene i...

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Abstract

The invention discloses a method for detecting DNA mutation (SNP and DNA methylation) based on graphene sensor. The method comprises the steps that: (1) a graphene molecular device is constructed; (2) a connection molecule is assembled on the surface of the graphene molecular device obtained in the step (1); (3) the designed detection probe is connected on the assembled molecule; and (4) a DNA mutation sample is subjected to electrical signal detection based on the graphene device. According to the invention, DNA mutation detection is realized through electrical signals by using the functionalized graphene molecular device.

Description

technical field [0001] The invention is a method for specifically detecting DNA mutation (single nucleotide polymorphism and DNA methylation), which belongs to the detection method research in the field of biology. Background technique [0002] The detection of DNA mutations (single nucleotide polymorphism and DNA methylation) currently exists, in which single nucleotide polymorphism is SNP, which is a mutation of a base in the DNA sequence, and DNA methylation detection is mostly DNA-based bisulfite treatment can convert non-methylated cytosine to uracil, while methylated cytosine does not change. This difference in mutant sequence can be detected by various methods . Most of these existing methods still have certain limitations in terms of detection sensitivity and specificity. [0003] In 2004, two scientists from the University of Manchester, Andre Geim and Konstantin Novoselov, prepared for the first time a new type of two-dimensional carbon nanomaterial - graphene. ...

Claims

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Application Information

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IPC IPC(8): G01N27/00
Inventor 高力周阳陈克平张春霞陈亮连超群
Owner JIANGSU UNIV
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