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Methods for preparing entomogenous fungus conjugate and extracting polysaccharides of entomogenous fungus conjugate

An extraction method and a combination technology, which are applied in botany equipment and methods, horticulture, applications, etc., can solve problems such as death, animal carcasses need to be processed, and animal immunity decline, so as to achieve the effect of increasing polysaccharide content and increasing economic value

Inactive Publication Date: 2015-03-18
NANYANG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after repeated stimulation, the immune ability of animals decreased, resulting in a large number of deaths, resulting in a large number of animal carcasses to be disposed of

Method used

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  • Methods for preparing entomogenous fungus conjugate and extracting polysaccharides of entomogenous fungus conjugate

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Experimental program
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preparation example Construction

[0016] (2), preparation of the conjugate: according to the ratio of 5~60ml: 200~300 of bacteria solution and centipede body number, the bacteria solution is evenly sprayed on the surface of the insect body with a watering can or the insect body is soaked in the bacteria solution for 1~ After 3 minutes, place it in an environment of 15-25°C and incubate for 7-15 days. After 3 days, the worms will appear and start to turn black. After 5 days, the hyphae will start to white mycelium. The body was dried naturally to obtain the combined product of centipede and Cordyceps sinensis; polysaccharide determination was carried out.

[0017] The above-mentioned Cordyceps sinensis can be purchased from the strain resource bank.

[0018] The method for extracting polysaccharides of insect-bacteria complexes of the present invention comprises the following steps:

[0019] (1), dissolving: get the bacterium powder of 50g dry insect-bacteria combination, add deionized water 3L by bacterium po...

Embodiment 1

[0023] Prepare materials according to the following quantity ratio

[0024] Bacterial solution 5mL

[0025] The number of centipede insects is 200

[0026] Spray the bacteria liquid evenly on the surface of the worm body with a watering can, then put it at 18°C, and cultivate it for 15 days. After 3 days, the worm body will appear and start to turn black. After 5 days, the hyphae will start to white mycelium. Take out the worm body and dry it naturally to obtain the combination product of centipede and Cordyceps sinensis.

Embodiment 2

[0028] Prepare materials according to the following quantity ratio

[0029] Bacteria liquid 5~15mL

[0030] Number of centipedes 200-300

[0031] Put the above-mentioned bacterial liquid and worms into the beaker, shake the beaker constantly, mix evenly, continue for 1 minute, then put it at 18°C, and cultivate it for 7 days. After 3 days, the mycelium begins to white mycelium, and after 5 days, it is covered with worms. After 7 days The worm body was taken out and dried naturally to obtain the combined product of centipede and Cordyceps sinensis, and the polysaccharide determination was carried out.

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Abstract

The invention discloses a method for preparing an entomogenous fungus conjugate from probiotics parasitic centipedes and a method for extracting the polysaccharides of the entomogenous fungus conjugate. The method for preparing the entomogenous fungus conjugate comprises the following steps of (1) culturing cordyceps sinensis; and (2) preparing the conjugate, namely mixing fungus liquid with the centipedes and culturing the mixture to obtain the conjugate of the centipedes and the cordyceps sinensis, and determining the polysaccharides. The method for extracting the polysaccharides of the entomogenous fungus conjugate comprises the steps of (1) dissolution; (2) extraction; and (3) washing, re-concentration, freeze-drying and crushing, so that the polysaccharides of the entomogenous fungus conjugate can be obtained. According to the technical scheme, a great quantity of polysaccharide substances can be generated by the obtained entomogenous fungus conjugate, electrically stimulated centipede bodies can be developed and utilized, the polysaccharide content can be improved, and the economic value of the centipede bodies is favorably improved.

Description

technical field [0001] The invention relates to the application of probiotic fungi, in particular to a method for preparing an insect-bacteria conjugate with probiotic parasitic centipedes and extracting polysaccharides from the conjugate. Background technique [0002] Centipedes are distributed in the north and south of my country. Traditional medicine has used centipedes in the prevention and treatment of diseases for more than two thousand years. The medicinal use was first seen in "Shen Nong's Herbal Classic". There are a pair of jaws and feet on the centipede's head that contain venomous glands that can secrete venom. On the one hand, these venoms penetrate through the venomous jaws and inject the venom into the prey animal to paralyze or even kill it. On the other hand, these venoms help strengthen the animal defenses. However, after the application of these venoms in medical research, it was found that these venoms have the effects of central nervous system inhibitio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C08B37/00
Inventor 夏西超丁可归改霞黄川峰马瑜红薛世鹏赵岩方家选
Owner NANYANG MEDICAL COLLEGE