Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection method of tissue distribution of small molecule polypeptide drugs

A technology of small molecule peptides and detection methods, which is applied in the field of detection of tissue distribution of small molecule peptide drugs, and achieves the effect of strong specificity and perfect test techniques and methods

Inactive Publication Date: 2013-10-02
TIANJIN INSTITUTE OF PHARMA RESEARCH
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many studies on the tissue distribution of chemical drugs and macromolecular proteins, and the methods are relatively mature. However, there is no relatively mature and universal method for the detection of tissue distribution of small molecule polypeptide drugs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of tissue distribution of small molecule polypeptide drugs
  • Detection method of tissue distribution of small molecule polypeptide drugs
  • Detection method of tissue distribution of small molecule polypeptide drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] In this example, the method of combining cell lysate extraction, liquid nitrogen cryopreservation and radioimmunoassay (RIA) established by the present invention was used to conduct a tissue distribution detection study of triptorelin acetate microspheres for injection in rats. The specific detection method is as follows:

[0032] 1. Triptorelin acetate microspheres for injection were provided by Changchun Jinsai Pharmaceutical Co., Ltd. Specifications: 3.75mg / bottle, containing freeze-dried powder (28-day sustained release) and special solvent (2mL). The batch numbers are lyophilized powder 20091104, valid until 20111103; special solvent 20090601, valid until 20110531. Store below 25°C. Triptorelin acetate was labeled with the chloramine T method (Ch-T) to prepare 125 I labeled triptorelin acetate freeze-dried product.

[0033] Triptorelin acetate was labeled with the chloramine T method (Ch-T method) to prepare 125 I labeled triptorelin acetate freeze-dried produc...

Embodiment 2

[0051] In this example, the method of combining cell lysate extraction, liquid nitrogen cryopreservation and enzyme-linked immunoassay (ELISA) established by the present invention was used to study the tissue distribution of recombinant human thymosin α1 in rats. The specific research methods are as follows:

[0052] 1. Recombinant human thymosin α1 (rh-Tα1), provided by Shenzhen Haiwang Pharmaceutical Co., Ltd., batch number: 20050301, the sample is white freeze-dried powder injection, purity 98.51%, content: 1.5mg / bottle; before the test, the sample was stored at 4°C save.

[0053] 2. ELISA detection kit for rh-Tα1, provided by Beijing Wanger Biotechnology Co., Ltd., stored at 2-8°C. Model 550 micro-plate reader (Model 550 Micro-plate reader) is a product of Bio-red Company in the United States.

[0054] 3. Experimental animals: 24 Wistar rats, both male and female, weighing 180±10 grams, provided by the Department of Experimental Animal Science, Peking University Health S...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a detection method of tissue distribution of small molecule polypeptide drugs. The detection method comprises the following steps of: preparing homogenate extracting solutions of different organs / tissues of an experimental animal by adopting key technologies such as cell lysate extraction and liquid nitrogen frozen storage; according to the properties and characteristics of drugs to be detected, taking an immunological assay determination technology, namely a radioimmunoassay (RIA) method or an enzyme-linked immunoassay (ELISA) method, as a major quantitative assay means.

Description

technical field [0001] The invention belongs to the technical field of life sciences, and relates to a method for detecting the tissue distribution of small-molecule polypeptide drugs, specifically, to a method for detecting small-molecule peptides by combining cell lysate extraction, liquid nitrogen cryopreservation, and immunological analysis and determination techniques. Method for tissue distribution of polypeptide drugs. Background technique [0002] In recent years, with the development of genomics, proteomics and biotechnology, various small-molecule polypeptide drugs with similar structures and functions to endogenous active proteins or peptides have emerged, involving the prevention and diagnosis of diseases. And treatment, etc., especially in many fields such as cancer, autoimmune diseases, and metabolic diseases. Compared with traditional small-molecule chemical drugs, small-molecule polypeptide drugs have the advantages of strong biological activity and small do...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/534G01N33/535
Inventor 蔡永明张宗鹏申文晋宋紫辉李春雨王海荣项宗尚张春云
Owner TIANJIN INSTITUTE OF PHARMA RESEARCH