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A class of cyanine compound, its preparation method and application

A technology of cyanine compounds and compounds, applied in chemical instruments and methods, flow characteristics, measuring devices, etc., can solve the problems of not being able to monitor mitochondrial viscosity in real time

Active Publication Date: 2015-08-12
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, this traditional method needs to centrifuge mitochondria in cells, and then calculate the mitochondrial viscosity through a series of tests, and cannot monitor the viscosity of mitochondria in living cells in real time.
[0004] At present, fluorescent probes for monitoring the viscosity of intracellular subcellular organelles are still rare, and fluorescent probes with two-photon properties that simultaneously monitor the viscosity of subcellular organelles through two modes of ratio fluorescence and fluorescence lifetime are even rarer, so research and It is of great significance to develop fluorescent probes capable of two-photon performance and simultaneously monitor the viscosity in subcellular organelles through dual modes

Method used

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  • A class of cyanine compound, its preparation method and application
  • A class of cyanine compound, its preparation method and application
  • A class of cyanine compound, its preparation method and application

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Experimental program
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Embodiment Construction

[0021] The cyanine compounds used for monitoring the viscosity value of subcellular organelles according to the present invention have the structure of general formula I:

[0022]

[0023] in,

[0024] In general formula I, R 1 from C 1-18 Alkyl, N(R 3 ) 2 , morpholino, substituted or unsubstituted phenyl; preferably C 1-18 Alkyl or benzyl; more preferably benzyl; most preferably, R 1 It is benzyl, which is directly connected to N, that is, n=0.

[0025] In general formula I, R 2 selected from H, SO 3 R 3 and COOR 3 , where R 3 from H and C 1-18 Alkyl; preferably R 2 for H.

[0026] Y - for Cl - , Br, I - or OTs - ;

[0027] n is an integer of 0-18.

[0028] Most specifically, the pentamethylcyanine compound substituted with a median aldehyde group in the present invention is BFT:

[0029]

[0030] That is, in the compound of general formula I, R 1 is benzyl, R 2 for H, n=0, Y - for Br - .

[0031] The applicant believes that those skilled in the...

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Abstract

The invention discloses a cyanine compound and a preparation method and an application thereof. The compound has a structure as shown in the general formula I. The probe is especially sensitive to environmental viscosity and can be used in detection of fluids and biotic environmental viscosity. The probe can be positioned at different subcellular organelles and can monitor viscosity changes in subcellular organelles in living cells by a bimodal detection means of fluorescence ratio and fluorescence lifetime.

Description

technical field [0001] The invention relates to a class of fluorescent probe compounds suitable for viscosity detection in subcellular organelles in living cells in the field of fine chemical industry. This type of compound can simultaneously monitor the viscosity value in subcellular organelles in living cells through two methods of fluorescence ratio and fluorescence lifetime. Background technique [0002] Intracellular viscosity plays an important role in biological processes such as signaling, nuclear functionalization, chromatin localization, and singlet oxygen localization. Sudden changes in intracellular viscosity lead to associated functionalization defects and disease. Mitochondria are the energy-making factories in cells, which are sensitive to cancer, Alzheimer's, Parkinson's and other diseases. The reduction of mitochondrial membrane viscosity, the weakening of electron transport chain activity, the increase of active oxygen content, and the increase of cytochr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D277/64C09B23/08C09K11/06G01N11/00
Inventor 彭孝军姜娜樊江莉王静云
Owner DALIAN UNIV OF TECH