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Application of Chukrasone B in medicine for treating lung cancer

A drug and lung cancer technology, applied in the field of anti-tumor drugs, can solve the problem of damage to normal tissues and cells, and achieve the effect of strong cytotoxicity and prominent substantive characteristics

Inactive Publication Date: 2013-11-27
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Malignant tumors are diseases that seriously threaten human life and health. Chemotherapy drugs commonly used in clinical practice can greatly damage normal tissues and cells while killing tumor cells, which limits its application.

Method used

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  • Application of Chukrasone B in medicine for treating lung cancer
  • Application of Chukrasone B in medicine for treating lung cancer
  • Application of Chukrasone B in medicine for treating lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: the preparation of the compound Chukrasone B tablet involved in the present invention:

[0013] Take 20 grams of compound Chukrasone B, add 180 grams of conventional excipients for tablet preparation, mix well, and make 1000 tablets with a conventional tablet press.

Embodiment 2

[0014] Embodiment 2: the preparation of the compound Chukrasone B capsules involved in the present invention:

[0015] Get 20 grams of compound Chukrasone B, add conventional auxiliary materials for preparing capsules such as 180 grams of starch, mix well, and pack into capsules to make 1000 tablets.

[0016] The following pharmacodynamic experiments will further illustrate its drug activity.

experiment example

[0017] Experimental example: the inhibitory effect of Chukrasone B on the proliferation of lung cancer cells cultured in vitro and the effect on normal human cells

[0018] Lung cancer cells in the logarithmic growth phase (non-small cell lung cancer cells A 549 , large cell lung cancer cells NCI-H460, lung squamous cell carcinoma cells HTB-8 and human lung squamous cell carcinoma cells QG-5) and human normal liver cells L02 were inoculated on 96-well culture plates, and the seeding volume per well was 3000-4000 cells / 100uL and 6000 cells / 100uL; aseptically collect 15mL of venous blood from healthy blood donors, anticoagulate with heparin, separate mononuclear cells by Ficoll density gradient centrifugation, prepare single cell suspension with RPMI1640 containing 10% fetal bovine serum, adjust Cell concentration was 2 x 10 5 cells / mL, inoculated in 96-well culture plate, 100uL per well. After 24 hours of cell culture on the above-mentioned culture plates, the experimental gr...

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PUM

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Abstract

The invention discloses application of Chukrasone B in preparation of a medicine for treating lung cancer, and belongs to the technical field of new application of medicines. The compound shows high lung cancer inhibition activity and can be used for preparing the medicine for treating lung cancer. The in-vitro MTT (3-4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) antitumor activity evaluation shows that Chukrasone B has remarkable inhibition effects on in vitro cultured non-small cell lung cancer cell A549, large cell lung cancer cell NCI-H460, lung squamous cancer cell HTB-8 and human lung squamous cell carcinoma cell QG-5, but has little inhibition effect on human normal hepatocyte LO2 and peripheral blood lymphocyte, so that Chukrasone B has the characteristic of obvious selectivitya. The application of Chukrasone B in the preparation of the medicine for treating lung cancer is disclosed for the first time; the framework type of Chukrasone B is brand new; Chukrasone B brings unexpected inhibition activity for lung cancer cells.

Description

technical field [0001] The invention relates to the field of antitumor drugs, in particular to the application of Chukrasone B in the preparation of antitumor drugs. Background technique [0002] Malignant tumors are diseases that seriously threaten human life and health. Chemotherapy drugs commonly used in clinical practice can greatly damage normal tissues and cells while killing tumor cells, which limits its application. It has become the direction and urgent task of current anticancer drug research to find highly efficient and low toxic drugs for treating tumors. Finding high-efficiency, low-toxic anticancer drugs has been considered an effective way. [0003] The present invention has confirmed by experiments that Chukrasone B has the effect on non-small cell lung cancer cell A cultured in vitro. 549 , large cell lung cancer cell line NCI-H460, lung squamous cell carcinoma cell line HTB-8 and human lung squamous epithelial cell line QG-5 have significant inhibitory ef...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61P35/00
Inventor 吴俊华刘洋黄蓉王慧张广江春平
Owner NANJING UNIV