Sponge material of collagen from freshwater fish and preparation method thereof

Abstract

The invention discloses a sponge material of collagen from freshwater fish and a preparation method thereof. The method comprises the following steps: 1) dissolving collagen of freshwater fish in a solution of diluted acid to obtain a collagen solution; 2) adding a phosphate buffer solution into the collagen solution, and regulating the pH value to 6.0-6.5; 3) performing ultrasound process, regulating the pH value of the processed solution to 7.0-7.6, and placing the solution in water baths at the temperature of 25-33 DEG C and 20-28 DEG C, each for 2-10 hours respectively, to obtain muddy collagen colloid; 5) performing centrifugation process on the muddy collagen colloid to obtain concentrated collagen colloid; 6) pouring the concentrated collagen colloid into a mold after cleaning and centrifugation, and performing ultrasound process and freeze drying to obtain the sponge material of the collagen from freshwater fish. The sponge material of the collagen prepared through the method provided by the invention has a higher density; collagen molecules are tightly combined with one another in the form of reorganized fiber; the product has a better performance consistency and in vivo stability.

Description

technical field [0001] The invention relates to a freshwater fish source collagen sponge material and a preparation method thereof, belonging to the technical field of medical biomaterials. Background technique [0002] Collagen is one of the most abundant and widely distributed protein types in multicellular organisms. In organisms, collagen, polysaccharides and other components together form a precise and orderly intercellular network structure——extracellular matrix (ECM), which plays an important role in the development and migration of body cells, as well as the formation and function of body tissues. play an important role. At present, there are more than 27 kinds of collagen known, which are named as type I, type II, type III and so on. Because collagen has good biocompatibility and can promote the growth, migration and proliferation of body cells, it is widely used in the fields of biomedical materials, medical tissue engineering and medical cosmetology. Such as th...

AI Technical Summary

Problems solved by technology

Nevertheless, compared with mammalian collagen, the freshwater fish collagen sponge material, which is used for rapid hemostasis, wound repair and cavity filling, still has obvious differences in thermal stability, in vivo degradation stability and in vivo material morphological stability. Disadvantages: For example, thermal denaturation, loss of inherent shape of the material after water absorption and swelling, and rapid degradation in the body are prone to occur under human body temperature conditions, and its biological functions cannot be fully exerted

Due to the limitation of the solubility of collagen, the collagen solution or collagen gel before freeze-drying contains a large amount of water or solvent, resulting in a low density of the freeze-dried collagen sponge material and easy to form large, non-uniform sponge pores, so the product machinery Poor performance and problems such as easy degradation, rapid swelling and loss of the original shape of the sponge, and small cell attachment area during use

[0005] Due to the use of cross-linking technology to modify the properties of collagen sponge materials, there are problems of one kind or another: for example, physical cross-linking methods (thermal cross-linking, ultraviolet cross-linking, etc.) often have unsatisfactory effects or lead to triple helix structure of collagen molecules. However, chemical cross-linking has problems such as chemical reagent residue or the release of chemical reagents after the collagen material is degraded in vivo. Therefore, fiber recombination technology has become an ideal technical means to improve the performance of collagen sponge materials.

In the above-mentioned published methods of utilizing fiber recombination technology to manufacture collagen sponge materials, a single condition is used to carry out collagen fiber reorganization in vitro, and then directly freeze-dried to obtain collagen sponge materials. Not only is it difficult to obtain an ideal restructured fiber particle size, but also The fiber particle size distribution range is wide, and some collagen still exists in the form of monomer molecules or small molecules such as microfibers, which leads to the inconsistency of the overall performance of collagen sponge products

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