Bacillus pumilus with efficient alga-lysing activity and application thereof
A Bacillus pumilus, applied technology, applied in the direction of bacteria, microbial-based methods, water/sludge/sewage treatment, etc., can solve high-cost, high-difficulty problems, and achieve good environmental compatibility, great competitiveness, and Effects of remediation control and governance
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Embodiment 1
[0025] Embodiment 1. has the screening of alginolytic activity bacillus strain
[0026] After activating 17 Bacillus strains with biocontrol ability isolated from soil samples in Tibet in the early stage of the laboratory, pick a single colony with a toothpick, inoculate it into 20ml LB medium, and culture it at 37°C and 200rpm for 24h; 200 μl was placed in 20 ml Landy medium, cultured and fermented at 30° C. with shaking at 200 rpm for 38 hours. Add 150 μl of fermentation broth to 30ml of Microcystis aeruginosa algae liquid in the logarithmic growth phase, and then place it in a light incubator with light intensity of 2500lx, temperature of 28°C, and light-dark ratio of 16h:8h for static cultivation. Shake 2 times. After 7 days, the chlorophyll-a content of each treatment was measured, the algae inhibition rate was calculated, and the statistical software of SPSS was used for analysis of variance statistics. The results show that: 17 Bacillus strains have different degrees ...
Embodiment 2
[0028] Embodiment 2. Exploration of the mechanism of inhibiting algae
[0029] The Bacillus fermentation liquid was centrifuged at 12000 rpm at 4°C for 10 min, and the supernatant was collected, which was the fermentation supernatant. Add sterilized water to the centrifuged bacteria to restore the original volume, namely the bacteria suspension. Inoculate 150 μL of fermentation broth, cell suspension, and fermentation supernatant into 30 mL of Microcystis aeruginosa algae liquid, and carry out algae-dissolving experiments with Landy medium as the control control. After 7 days, measure the content of chlorophyll a, and calculate the algae inhibition rate. To compare the effects of different treatments of fermentation broth on Microcystis aeruginosa. Results: The algae inhibition rate of the fermentation supernatant was 98.0%, while that of the bacterial suspension was only 10.23% (see image 3 ). It indicated that Bacillus pumilus NMCC46 played the role of algae lysis mainly...
Embodiment 3
[0030] Embodiment 3. Effects of different fermentation supernatant concentrations on the growth of Microcystis aeruginosa
[0031]In order to study the algicidal effect of different concentrations of Bacillus pumilus NMCC46 fermentation supernatant on Microcystis aeruginosa, and to find the best dosage of fermentation supernatant, we took 300μl, 150μl, 30μl, 15μl of fermentation supernatant and added them to 30ml In the Microcystis aeruginosa liquid, the final concentration was 1%, 5‰, 1‰, and 0.5‰ in four gradients, and the treatment without adding the fermentation supernatant was used as the control. The content of chlorophyll a was measured by 90% acetone extraction every 1 day, and their algalytic effect was judged by continuously observing the change of chlorophyll a content during 7 days of treatment. Results: The fermentation supernatant had a certain inhibitory effect on the growth of Microcystis aeruginosa at the tested concentrations. At the concentration of 1% and ...
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