Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sugarcane genome endogenous reference gene fructose-6-phosphate 2-kinase gene PCR (polymerase chain reaction) primer sequences and amplification method

An internal standard gene and primer sequence technology, applied in recombinant DNA technology, DNA/RNA fragments, DNA preparation, etc., can solve the problem of no research report on copy number, and achieve the effect of easy determination, wide applicability and strong specificity

Inactive Publication Date: 2013-11-27
FUJIAN AGRI & FORESTRY UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also many studies on the fructose-6-phosphate 2-kinase gene that produces this enzyme, but there is no research report on the copy number of this gene in the genome of sugarcane species

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sugarcane genome endogenous reference gene fructose-6-phosphate 2-kinase gene PCR (polymerase chain reaction) primer sequences and amplification method
  • Sugarcane genome endogenous reference gene fructose-6-phosphate 2-kinase gene PCR (polymerase chain reaction) primer sequences and amplification method
  • Sugarcane genome endogenous reference gene fructose-6-phosphate 2-kinase gene PCR (polymerase chain reaction) primer sequences and amplification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) PCR amplification of sugarcane fructose-6-phosphate 2-kinase gene

[0022] Fructose-6-phosphate 2-kinase gene PCR primer sequence is: ScF2KP -F:CCTGGGAGGATTGTCTTCTTC; ScF2KP -R: ATCACCACCGATTCTTCCTCT.

[0023] PCR reaction system: 1.0 U Taq DNA polymerase, 1×PCR buffer solution, 0.25 mM dNTP, 3.0 mM MgCl 2 , primer ScF2KP -F and ScF2KP Each of -R was 0.25 mM, DNA template was 25 ng, and the total volume of the reaction system was 25 μl.

[0024] PCR amplification conditions: pre-denaturation at 95°C for 6 min; denaturation at 94°C for 30 s; annealing at 56°C for 30 s, extension at 72°C for 35 s, a total of 35 cycles, and a final extension at 72°C for 7 min.

[0025] (2) Electrophoresis detection of PCR product of sugarcane fructose-6-phosphate 2-kinase gene and verification of product specificity and universality

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses sugarcane genome endogenous reference gene fructose-6-phosphate 2-kinase gene PCR (polymerase chain reaction) primer sequences and an amplification method. The primer sequences are composed of ScF2KP-F and ScF2KP-R. The PCR amplification method comprises a reaction system and amplification conditions. The invention provides a genome endogenous reference gene for PCR detection and species specific identification of transgenic sugarcane, and meanwhile, provides insurance for detection precision and result accuracy of the transgenic sugarcane.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a PCR primer sequence and an amplification method of an internal standard gene fructose-6-phosphate 2-kinase gene in sugarcane genome. Background technique [0002] Transgenic technology is becoming more and more mature, and the types of genetically modified sugarcane are becoming more and more diverse. At present, herbicide-resistant, insect-resistant, and disease-resistant genetically modified sugarcane has been field tested in 14 countries including the United States, Australia, India, and Brazil, creating conditions for the commercial cultivation of genetically modified sugarcane. In my country, sugarcane gene cloning and transgenic research only started in the mid-1990s. Although it started late, it has made rapid progress. The transgenic sugarcane that has been obtained includes insect-resistant genetically modified sugarcane, drought-resistant genetically m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/11C12N15/10
Inventor 陈平华林冰陈忠伟陈利平施桂姣张卓项慰刘迪夏峰王恒波高三基许莉萍陈如凯
Owner FUJIAN AGRI & FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com