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Method used for producing bioethanol with Scenedesmusabundans

A technology of Scenedesmus and ethanol, which is applied in the field of biomass energy and fermentation engineering, and can solve problems such as the limitation of biomass energy development and the occupation of land resources

Inactive Publication Date: 2013-12-04
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, whether it is the first generation or the second generation of bioenergy, there are restrictions on the need to occupy land resources. Due to the scarcity of land resources, terrestrial biomass is used as the Basic biomass development is limited

Method used

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Embodiment Construction

[0008] The operation process of the present invention comprises: microalgae Scenedesmus abundans Biomass expanded cultivation, yeast cultivation, saccharification, fermentation.

[0009] 1. Microalgae Scenedesmus abundans Biomass Expansion Cultivation

[0010] Add 10L of BG11 (pH=7.5) medium into a 15L photoreactor and inoculate into algae S.abundans . Using the method of semi-continuous culture, when the microalgae grow to the end of the logarithmic growth phase or the beginning of the stable phase, part of the biomass is harvested. The harvested biomass is mainly the lower part of the sediment, and the remaining biomass continues to be cultured and supplemented into a new BG11 culture. base. Centrifuge at 5000rpm for 5 minutes and remove the supernatant. Wash twice with distilled water to remove the culture medium of the algal species and other soluble impurities, centrifuge to remove the supernatant, freeze-dry in a freeze dryer for about 24 hours until compl...

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Abstract

The invention provides a method used for producing bioethanol by saccharomyces cerevisiae fermentation, wherein Scenedesmusabundans is taken as biomass. The method comprises steps of cultivation of microalgae biomass, saccharification and fermentation. In the step of cultivation of microalgae biomass, the biomass of Scenedesmusabundans is enlarged by illumination cultivation in BG11 medium under a constant temperature. In the step of saccharification, Scenedesmusabundans biomass obtained in the step above is treated by acid, cellulase and glucoamylase so as to release saccharides such as cellulose and starch in the Scenedesmusabundans biomass, and transform the saccharides into glucose. In the step of fermentation, the biomass treated by saccharification is inoculated with saccharomyces cerevisiae, and bioethanol is produced in a constant temperature shaking table under amaerobic conditions.

Description

technical field [0001] The invention relates to biomass energy and fermentation engineering technology, specifically utilizing microalgae enriched Scenedesmus ( Scenedesmus abundans) As biomass, through Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) fermentation to produce biomass ethanol. Background technique [0002] With the increasing demand for energy brought about by human activities, the increasing shortage of petrochemical energy, and many environmental problems brought about by the exploitation and utilization of petrochemical energy, people have to develop new alternative energy to solve the problem of petrochemical energy crisis. . [0003] Biomass energy has been widely concerned as a new energy source. After decades of development, three generations of bioenergy forms have emerged. The first generation of bioenergy refers to energy substances produced by using traditional extraction techniques using starch, sugar, animal fat and vegetable oil as r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12P19/14C12R1/865
CPCY02E50/17Y02E50/10
Inventor 郭慧汪光义
Owner PEKING UNIV SHENZHEN GRADUATE SCHOOL