Analyzing method for continuous cropping peanut soil eukaryotic microbial community succession
A technology of microbial community and analysis method, which is applied in the field of analysis of the succession of eukaryotic microbial communities in continuous cropping peanut soil, can solve the problem of not analyzing the succession law of microbial groups in continuous cropping peanuts, and achieve the effect of short cycle, high efficiency and comprehensive display.
Active Publication Date: 2015-05-06
SHANDONG PEANUT RES INST
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Problems solved by technology
However, there is currently no effective method for analyzing the succession of specific microbial groups during peanut continuous cropping.
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[0015] The technical solution of the present invention is further illustrated below through specific examples.
[0016] A method for analyzing the succession of eukaryotic microbial communities in peanut soil. Pot experiments were used to select soil that had not been planted with peanuts, mix well, distribute to each pot, and plant peanuts for three consecutive years. Sow seeds at the end of April and harvest at the end of September. No other crops are cultivated after the annual peanut harvest. During the planting process, a ventilated greenhouse is used to block rainwater, irrigated with sterile water, and no fertilizer is applied during the entire planting process to reduce the impact of the external environment on soil microorganisms, and reflect the interaction between peanut continuous cropping and soil micro-eukaryotes as much as possible. relation. Peanut root soil was collected at seedling stage and harvest stage in each planting cycle. In each period, three peanu...
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Abstract
The invention relates to an analyzing method for continuous cropping peanut soil eukaryotic microbial community succession. The method includes adopting a pot experiment, selecting soil free of planting peanuts, mixing the soil complete uniformly and dividing to pots, and planting the peanuts for three years continuously. Times for sowing and harvesting in the three year are consistent. In each planting period, soil at peanut roots are collected in the period of seedling and the period of harvesting, soil genomic DNA are extracted, and 18SrRNA gene libraries are built. By analyzing and comparing the built libraries, laws of the continuous cropping peanut soil eukaryotic microbial community succession can be acquired accordingly. By the aid of the analyzing method, community structure and dominant communities of continuous cropping peanut soil eukaryotic microbial community can be definite, and the succession laws in the process of peanut continuous cropping of each eukaryotic microbial community can be acquired.
Description
technical field [0001] The invention relates to a method for analyzing the succession of eukaryotic microbial communities in soil for continuous cropping peanuts, and belongs to the technical field of soil microorganisms and crop cultivation. Background technique [0002] Peanut is an important oil crop and economic crop in the world with wide adaptability, but its growth and yield are seriously affected by continuous cropping. Soil microorganisms play an important role in maintaining soil fertility and the stability of soil ecological functions due to their participation in processes such as the cycle of minerals in the soil, the degradation of organic matter, the formation of soil structure, and the transformation and accumulation of toxic substances. In addition, the interaction between pathogenic bacteria and antagonistic bacteria in soil also plays a very important role in the growth and development of plants. Because microorganisms can respond rapidly to environmental...
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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 陈明娜杨庆利潘丽娟迟晓元杨珍陈娜王通王冕禹山林
Owner SHANDONG PEANUT RES INST