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Novel feed containing PHA cell

A polyhydroxyalkanoate and feed technology, applied in animal feed, animal feed, application and other directions, can solve the problems of affecting technology promotion and high cost, and achieve the effects of improving nutritional value, promoting growth, and avoiding use.

Inactive Publication Date: 2013-12-11
TIANAN BIOLOGIC MATERIAL NINGBO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent with application number 201110071523.1 describes the enhanced method of feeding Artemia with purified PHB powder. Since the purified PHA powder is used, the cost is very high, which affects the promotion of the technology.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] After the Artemia eggs are hatched, collect the nauplii and transfer them to a shaking flask filled with 500ml of seawater. The density of the larvae is about 30,000 / L. Add 0.05ml of fermentation broth (the fermentation time is 30 hours, and the dry weight of cells is 50g / L). L, PHB content is 40%), intensive culture for 26 hours (PHB concentration is about 10mg / L), collect Artemia, wash with distilled water three times and then dry at 70 degrees, the measured PHB content in dried Artemia is 1.11% .

Embodiment 2

[0021] After the Artemia eggs are hatched, collect the nauplii and transfer them to a shaker flask filled with 1000ml of seawater. The density of the larvae is about 20,000 / L. Add 2.22g of fermented liquid dry powder (fermentation time of the fermented liquid is 120 hours, and the dry weight of the cells is 210 grams. / L, PHB content is 90%), intensive culture for 32 hours (PHB concentration is about 2000mg / L), collect Artemia, wash three times with distilled water and dry at 60 degrees, the measured PHB content in dried Artemia is 11.83 ﹪.

Embodiment 3

[0023] After the rotifer eggs are hatched, collect the nauplii and transfer them to a shaker flask filled with 1000ml of seawater. The density of the larvae is about 15000 / L. , the PHBV content is 80% and the HV content is 3.5%), intensive culture for 32 hours (PHBV concentration is about 136mg / L), collect rotifers, wash with distilled water three times and then dry at 60 degrees to measure the PHBV content in rotifers It is 5.85%.

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Abstract

The invention discloses novel feed containing a PHA (polyhydroxyalkanoate) cell. The feed is a nonselective filter-feeding aquatic animal containing PHA, wherein the content of PHA is 1-12%; a PHA fermentation stock solution is directly fed to the nonselective filter-feeding aquatic animal serving as biological bait, or powder formed by drying the PHA fermentation stock solution is directly fed to the nonselective filter-feeding aquatic animal serving as the biological bait, so that a purpose of biologically wrapping PHA and other nutrient substances is achieved; and the nonselective filter-feeding aquatic animal feed with the content of PHA of 1-12% is formed. The feed can facilitate growth of the aquatic animal, can improve the immunity of the aquatic animal, and can avoid use of antibiotics, so that a nutrient value of the aquatic animal is increased.

Description

technical field [0001] The invention belongs to the field of aquatic animal breeding, and relates to a novel feed containing polyhydroxyalkanoate (PHA) cells, in particular to the use of non-selective filter-feeding aquatic animals to biologically coat microbial cells containing polyhydroxyalkanoate (PHA) The product of the product is used as a feed, specifically, the fermentation broth of cells containing polyhydroxyalkanoate or its dry matter is directly fed to non-selective filter-feeding aquatic animals that are usually used as bait, so as to achieve concentration and transfer nutritional enhancement substances through the food chain The purpose of PHA and other nutrients. Background technique [0002] PHA is a biopolymer that uses glucose as the main raw material and is produced by special microbial fermentation engineering technology. Fermentative strains producing polyhydroxyalkanoate (PHA) by fermentation usually include Alcaligenes rosenbergii ( Ralstonia eutropha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/18A23K1/175
Inventor 凌东鹰沈华陈学军
Owner TIANAN BIOLOGIC MATERIAL NINGBO