Application of chrysin to preparation of drugs for treating obesity-related metabolically triggered inflammations
A technology of chrysin and obesity, applied in the direction of metabolic diseases, antipyretics, anti-inflammatory agents, etc.
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[0019] 1. Activity detection of chrysin in macrophages
[0020] The macrophage cell line ANA-1 in the logarithmic growth phase was selected, centrifuged and diluted with DMEM medium to 5*10 4 / mL, seeded in a 96-well plate. After culturing overnight at 37°C, add different concentrations of chrysin (10 μM, 20 μM, 50 μM, 100 μM), and set up a control group, incubate at 37°C in a 5% CO2 incubator for 24 hours, add 10 μL of MTT solution, incubate at 37°C for 4 hours, and measure with a microplate reader Absorbance at 570nm. The result is as figure 1 , found that the activity of chrysin in ANA-1 macrophages was dose- and time-dependent. When the drug concentration is 10μM, after 12 hours, there is a significant difference from the normal group. With the increase of concentration or time, the proliferation rate of cells decreases significantly.
[0021] 2. Chrysin inhibits typical activation of macrophages
[0022] 2.1 The effect of chrysin on the expression of Marker CD80 and...
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