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Application of chrysin to preparation of drugs for treating obesity-related metabolically triggered inflammations

A technology of chrysin and obesity, applied in the direction of metabolic diseases, antipyretics, anti-inflammatory agents, etc.

Inactive Publication Date: 2013-12-11
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the effect of chrysin on promoting the alternative activation of macrophages to inhibit inflammation

Method used

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  • Application of chrysin to preparation of drugs for treating obesity-related metabolically triggered inflammations
  • Application of chrysin to preparation of drugs for treating obesity-related metabolically triggered inflammations
  • Application of chrysin to preparation of drugs for treating obesity-related metabolically triggered inflammations

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Experimental program
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Embodiment Construction

[0019] 1. Activity detection of chrysin in macrophages

[0020] The macrophage cell line ANA-1 in the logarithmic growth phase was selected, centrifuged and diluted with DMEM medium to 5*10 4 / mL, seeded in a 96-well plate. After culturing overnight at 37°C, add different concentrations of chrysin (10 μM, 20 μM, 50 μM, 100 μM), and set up a control group, incubate at 37°C in a 5% CO2 incubator for 24 hours, add 10 μL of MTT solution, incubate at 37°C for 4 hours, and measure with a microplate reader Absorbance at 570nm. The result is as figure 1 , found that the activity of chrysin in ANA-1 macrophages was dose- and time-dependent. When the drug concentration is 10μM, after 12 hours, there is a significant difference from the normal group. With the increase of concentration or time, the proliferation rate of cells decreases significantly.

[0021] 2. Chrysin inhibits typical activation of macrophages

[0022] 2.1 The effect of chrysin on the expression of Marker CD80 and...

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Abstract

The invention belongs to the technical field of Chinese traditional medicine application, and specifically relates to application of chrysin to preparation of drugs for treating obesity-related metabolically triggered inflammations. According to the invention, the chrysin promotes the replacement activation of IL-4 activated mouse macrophages, promotes the release of anti-inflammatory cytokines IL-10, and promotes the rise of enzyme activity of typical labeled molecule arginine for the replacement activation of the macrophages, the up-regulation of surface molecules MGL1 / 2 and the up-regulation of classical molecules CD206, Ym1 and Fizz. Especially, in fat mice C57 / B6 induced by high-fat feeding, the chrysin is capable of inhibiting the release of proinflammatory cytokines TNF-alpha, AST (Aspartate Transaminase) and ALT (Alanine Transferase) in blood serum, increasing the release of the anti-inflammatory cytokines IL-10, alleviating the lesion of liver and adipose tissues, inhibiting the phagocytic function of the peritoneal macrophages, the antigen presentation ability and the release ability of NO, and lowering the expression of the typically activated surface molecules CCR7 and CD80 of the macrophages. According to the invention, a new drug for treating the obesity-related metabolically triggered inflammations is developed.

Description

1. Technical field [0001] The invention belongs to the technical field of traditional Chinese medicine application, and in particular relates to the application of chrysin in the preparation of a medicine for treating obesity-related metabolic inflammation. 2. Background technology [0002] Chrysin (Chrysin), chemical name 5,7-dihydroxybrass, is a natural flavonoid compound with a wide range of pharmacological effects extracted from the plant of Bignoniaceae. It is widely found in a variety of plants, propolis and honey Among them, it is the main component of propolis. Due to its wide range of pharmacological activities such as anti-oxidation, anti-tumor, anti-virus, anti-anxiety, anti-allergic and inhibiting aromatase activity, it has attracted much attention. [0003] The anti-inflammatory effect of chrysin mainly includes: inhibiting the allergic inflammatory response mediated by mast cells by blocking the release of histamine and the gene expression of pro-inflammatory ...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P3/04A61P29/00
Inventor 沈萍萍丰秀静秦浩瀚杨威威
Owner NANJING UNIV
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