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Composite micro-ecological preparation for nursery stock planting

A technology of compound microecology and preparation, applied in the directions of plant growth regulators, plant growth regulators, microorganism-based methods, etc., can solve the problems of slow growth and low survival rate of seedlings, so as to improve the survival rate, promote growth, ecological The effect of environmental cost

Inactive Publication Date: 2013-12-11
福州大用生物应用科技有限公司
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AI Technical Summary

Problems solved by technology

The planting and cultivation of seedlings is the primary link of greening. In the process of planting and cultivating seedlings, the growth of seedlings requires a large amount of nutrients, especially a variety of chemical elements. At present, it is mainly carbohydrates produced by photosynthesis and nutrients in the soil. To supply the needs of its growth, but in many cases, photosynthesis and nutrient content in the soil cannot meet the needs of seedling growth, resulting in slow growth of seedlings and relatively low survival rate

Method used

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Embodiment Construction

[0020] The present invention is a kind of compound micro-ecological preparation that is used for seedling planting, and the concrete steps of its preparation method are as follows:

[0021] (1) Slant culture of original strains: activate photosynthetic bacteria, nitrogen-fixing bacteria, silicate bacteria, cyanobacteria, and Saccharomyces cerevisiae in the cryopreservation tubes on the slant, and then place them in 90mm petri dishes for purification to obtain photosynthetic bacteria Slant strains, nitrogen-fixing bacteria slant strains, silicate bacteria slant strains, cyanobacteria slant strains, and Saccharomyces cerevisiae slant strains, for standby;

[0022] (2) Liquid culture of original strains: Inoculate the slant strains of photosynthetic bacteria into sterilized liquid medium Ⅰ, and then culture them under anaerobic tungsten light at 28°C-30°C for 5-7 days to obtain the original photosynthetic bacteria Bacterial solution: inoculate the slant bacteria of nitrogen-fixin...

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Abstract

The invention provides a composite micro-ecological preparation for nursery stock planting. A preparation method of the composite micro-ecological preparation comprises the following steps of activating photosynthetic bacteria, nitrogen-fixing bacteria, silicate bacteria, blue-green algae and saccharomyces cerevisiae in a freezing preservation tube respectively in a slant; purifying to obtain slant strains for later use; preparing the corresponding original bacterial liquid by use of each slant strain; inoculating the original bacterial liquids at a bacterial amount ratio of 2:2:2:2:1 in a sterilized fermentation medium I for culturing to obtain a first-level seed of the composite micro-ecological preparation; inoculating the first-level seed at an inoculation amount of 5% in a sterilized fermentation medium II for culturing to obtain a second-level seed of the composite micro-ecological preparation; and finally, inoculating the second-level seed at an inoculation amount of 5% in a sterilized fermentation medium III for culturing to obtain a finished product of the composite micro-ecological preparation. By adopting the composite micro-ecological preparation provided by the invention, the growth of the nursery stock can be promoted, and the survival rate of the nursery stock in planting culture can be improved; and moreover, the composite micro-ecological preparation also has the characteristics of ecological environmental protection and low cost.

Description

【Technical field】 [0001] The invention relates to a compound micro-ecological preparation for plant cultivation, in particular to a compound micro-ecological preparation for seedling planting. 【Background technique】 [0002] With the development of our country's ecological construction, afforestation is becoming more and more important in our country's ecological construction, and people's quality and requirements for greening are getting higher and higher. The planting and cultivation of seedlings is the primary link of greening. In the process of planting and cultivating seedlings, the growth of seedlings requires a large amount of nutrients, especially a variety of chemical elements. At present, it is mainly carbohydrates produced by photosynthesis and nutrients in the soil. To supply the needs of its growth, but in many cases, photosynthesis and nutrient content in the soil cannot meet the needs of seedling growth, resulting in slower growth of seedlings and relatively l...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/12C12N1/18A01N63/00A01N63/02A01P21/00C12R1/065C12R1/865C12R1/89
Inventor 陈琳莫艳华秦火保周世须
Owner 福州大用生物应用科技有限公司