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A visual rapid detection method for copper ions

A detection method and technology of copper ions, applied in the field of analytical chemistry, can solve the problems of inability to use actual samples for detection and low anti-matrix interference ability, and achieve good anti-matrix interference ability, good specificity, and simple operation

Inactive Publication Date: 2015-09-09
FUZHOU UNIV
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Problems solved by technology

These click chemistry reactions are catalyzed by Cu(I) complexes, which require the removal of oxygen to avoid oxidation and failure, thus limiting the application of click chemistry in the on-site detection of copper ions
At the same time, the above-mentioned rapid detection method also has the disadvantages of low anti-matrix interference ability and cannot be used for actual sample detection.

Method used

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  • A visual rapid detection method for copper ions
  • A visual rapid detection method for copper ions
  • A visual rapid detection method for copper ions

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Embodiment Construction

[0026] The present invention utilizes phosphatidylserine (DOPS) liposomes to Cu under specific pH 2+ With specific binding ability, with DOPS as Cu 2+ Recognition probe, combined with nano-gold to realize the recognition of Cu 2+ Visual quick detection. The main technical scheme is: using a double-layer membrane method to controlly assemble phosphatidylserine (DOPS) liposomes on the surface of nano-gold to prepare a solution of nano-gold modified by phosphatidylserine liposomes. The prepared DOPS-modified nano-gold solution was stable wine red, and at a certain pH, DOPS on the surface of nano-gold could specifically bind Cu 2+ (This process is reversible and controlled by pH) to aggregate the gold nanoparticles and produce a color change (with Cu 2+ The concentration increases, the color changes from wine red to purple to blue), reaching the Cu 2+ Visual quick detection.

[0027] The preparation method of the nano-gold test solution modified by DOPS is as follows:

[0...

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Abstract

The invention discloses a method for visually and quickly detecting copper ions based on phosphatidylserine (DOPS) liposome-modified nano-gold. DOPS is assembled on the surface of nano-gold by utilizing the high specific binding force of DOPS to Cu2+ ions at a certain pH , using two DOPS combined with a Cu2+ ion to reduce the distance between adjacent gold nanoparticles, so that the gold nanoparticles aggregate to produce a color change (from red to blue), so as to achieve visual and rapid detection of the concentration of Cu2+ ions. The method of the invention has the characteristics of rapidity, simplicity, sensitivity, visualization, high specificity, strong resistance to matrix interference, low cost and the like, and can be used for rapid and visual detection of copper ions in various water samples.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a method for visually and rapidly detecting copper ions, in particular to a method for visually and rapidly detecting copper ions based on phosphatidylserine (DOPS) liposome-modified nano gold. Background technique [0002] Copper, as a non-ferrous transition metal, is widely used in industrial and agricultural production processes. However, due to excessive development and utilization, the environment, especially the water environment, is seriously polluted by copper ions. For example, high concentrations of copper are often illegally added to pig and poultry feed to promote their growth, and high-copper pig manure is used as organic fertilizer on vegetable fields, thereby accumulating in the human liver through the food chain. Although copper ions are one of the essential elements for the human body, copper is highly toxic as a heavy metal element. Excessive copper intake ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N1/28
Inventor 付凤富杨玮娟何叶
Owner FUZHOU UNIV
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