Method for extracting active ingredients from coreopsis tinctoria by subcritical water

A technology of active ingredients and subcritical water, applied in solid solvent extraction and other directions, can solve the problems that active ingredients cannot be fully extracted and utilized, and the extraction rate is low, and achieves the effect of maintaining natural quality, high extraction efficiency and convenient operation.

Inactive Publication Date: 2014-01-01
XINJIANG UNIVERSITY
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  • Summary
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Problems solved by technology

But, because the content of the active component in the snow chrysanthemum is higher, and the stability under water and higher temperature is good, these above-mentioned methods exi

Method used

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  • Method for extracting active ingredients from coreopsis tinctoria by subcritical water

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Experimental program
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Effect test

Embodiment 1

[0027] After pulverizing 2g of Xinjiang snow chrysanthemum, place it in a 100mL extraction kettle of subcritical fluid extraction process, first add 10mL of distilled water to soak for 5min, and stir to make the snow chrysanthemum powder completely wet by water, then add 40mL of distilled water, seal the extraction kettle, and make The extraction temperature was raised to 120°C within 5 minutes, and the extraction was carried out for 15 minutes; the temperature of the extraction kettle was quickly lowered to below 50°C, the extraction kettle was opened, and the filtrate was collected by filtration; The temperature was raised to 150°C within 5 minutes, extracted for 15 minutes, filtered, and the filter residue was washed with a small amount of hot water, the filtrate was combined, and the filtrate was concentrated under reduced pressure to obtain 0.95 g of chrysanthemum extract with a total flavonoid content of 28.7%.

Embodiment 2

[0029] Weigh 5g of pulverized Xinjiang snow chrysanthemum samples and place them in a 100mL subcritical fluid extraction extraction kettle, add 20mL distilled water to soak for 5min, stir to make the snow chrysanthemum powder completely wet by water, then add 30mL distilled water, seal the extraction kettle, and make the extraction The temperature was raised to 100°C within 5 minutes, extracted for 1 hour, and the extraction kettle was quickly cooled to room temperature, filtered, and the filter residue was put into the extraction kettle and 40 mL of distilled water was added, and then extracted for 0.5 hours; cooled and filtered, the filtrates were combined twice, and concentrated to obtain 1.35 g snow chrysanthemum extract, in which the total flavonoid content of snow chrysanthemum is 41.6%.

Embodiment 3

[0031] Weigh 5g of pulverized Xinjiang snow chrysanthemum samples and place them in a 100mL subcritical fluid extraction extraction kettle, add 20mL distilled water to soak for 5min, stir to make the snow chrysanthemum powder completely wet by water, then add 30mL distilled water, seal the extraction kettle, and make the extraction The temperature was raised to 140°C within 5 minutes, extracted for 1 hour, and the extraction kettle was quickly cooled to room temperature, filtered, and the filter residue was put into the extraction kettle and 40 mL of distilled water was added, and the above conditions were repeated for another 0.5 hours; cooled and filtered, the filtrates were combined twice, and concentrated Obtain 1.85g snow chrysanthemum extract afterward, wherein the total flavonoid content of snow chrysanthemum is 32.7%.

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Abstract

The invention provides a method for extracting active ingredients from dry coreopsis tinctoria. The method comprises the following steps: adopting subcritical water as an extracting solvent for simultaneously extracting two active ingredients of coreopsis tinctoria flavones and coreopsis tinctoria polysaccharides from the coreopsis tinctoria by controlling the extraction temperature to be 100-250 DEG C and the extraction pressure to be 0.1-15 MPa for 0.5-2 hours; directly performing reduced pressure concentration on an extracting solution containing the active ingredients of the coreopsis tinctoria to obtain an extract containing coreopsis tinctoria flavones and coreopsis tinctoria polysaccharides simultaneously; or concentrating the extracting solution and separating out coreopsis tinctoria polysaccharides through alcohol precipitation to obtain a coreopsis tinctoria flavone extract with the total flavone content more than 50%. The various extracts can be applied to industries of medicines, food ingredients, beverage ingredients, healthcare tea ingredients, cosmetics and dyes. According to the method, the natural active ingredients in the coreopsis tinctoria are extracted by fully utilizing the change characteristics of the solvent properties of the subcritical water, and the requirements of green chemistry and chemical engineering are met.

Description

technical field [0001] The invention relates to a method for extracting bioactive components from Xinjiang snow chrysanthemum by using subcritical water, and belongs to the technical field of extraction of natural active components. More specifically, the present invention relates to a method of simultaneously extracting the two active components of snow chrysanthemum flavonoids and snow chrysanthemum polysaccharides from snow chrysanthemum by using dried Xinjiang snow chrysanthemum as raw material and using subcritical water as the extraction solvent, by controlling the corresponding extraction temperature and pressure. Components, the obtained aqueous solution containing the active ingredients of chrysanthemum flavonoids and chrysanthemum polysaccharides is concentrated under reduced pressure and separated by alcohol precipitation to obtain chrysanthemum polysaccharide extracts and high-content chrysanthemum flavonoids extracts. The chrysanthemum extracts are suitable for I...

Claims

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Application Information

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IPC IPC(8): B01D11/02
Inventor 刘玉梅祁英刘奎钫刘玉英
Owner XINJIANG UNIVERSITY
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