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Chemical modified fiber type immobilization carrier as well as preparation method and application thereof

An immobilized carrier and chemical modification technology, applied in the direction of biochemical equipment and methods, fixed on/in organic carriers, specific-purpose bioreactors/fermenters, etc., can solve the problem of long butanol fermentation cycle, Butanol needs to be improved and the production rate is low to achieve the effect of improving immobilization efficiency, cell immobilization efficiency and production efficiency

Inactive Publication Date: 2014-01-01
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are bottlenecks such as long fermentation period, low production rate, low conversion rate and low product concentration in the production of butanol by biological method
[0005] The current means of preparing butanol still needs to be improved

Method used

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  • Chemical modified fiber type immobilization carrier as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This example is to demonstrate the application of modified bagasse as an immobilized carrier in single-batch fermentation of butanol.

[0042] Bacteria: Clostridium acetobutylicum Clostridium acetobutylicum XY16 (CCTCC NO: M 2010011)

[0043] Seed medium: Seed medium: yeast powder 3 g / L, peptone 5 g / L, soluble starch 10 g / L, ammonium acetate 2 g / L, NaCl 2 g / L, MgSO 4 3 g / L, KH 2 PO 4 1 g / L, K 2 HPO 4 1 g / L, FeSO 4 ·7H 2 O 0.1 g / L, pH 6.0;

[0044] Fermentation medium (P2): carbon source (glucose 60 g / L dissolved), K 2 HPO 4 0.5 g / L, KH 2 PO 4 0.5 g / L, CH3 COONH 4 2.2 g / L, MgSO 4 ·7H 2 O 0.2 g / L, MnSO 4 ·H 2 O 0.01 g / L, NaCl 0.01 g / L, FeSO 4 ·7H 2 O 0.01 g / L; corn steep liquor 1 g / L.

[0045] In this experiment, sugarcane bagasse was taken as an example to illustrate the specific steps of selecting the dosage of polyethyleneimine modifier.

[0046] The amount of bagasse added was 10 g / L (modified by 4 g / L polyethyleneimine and 1 g / L glutaralde...

Embodiment 2

[0048] This example is to demonstrate the application of modified corn stalks as an immobilized carrier in single-batch fermentation of butanol.

[0049] Bacteria: Clostridium acetobutylicum Clostridium acetobutylicum XY16 (CCTCC NO: M 2010011)

[0050] Seed medium: Seed medium: yeast powder 3 g / L, peptone 5 g / L, soluble starch 10 g / L, ammonium acetate 2 g / L, NaCl 2 g / L, MgSO 4 3 g / L, KH 2 PO 4 1 g / L, K 2 HPO 4 1 g / L, FeSO 4 ·7H 2 O 0.1 g / L, pH 6.0;

[0051] Fermentation medium (P2): carbon source (glucose 60 g / L dissolved), K 2 HPO 4 0.5 g / L, KH 2 PO 4 0.5 g / L, CH 3 COONH 4 2.2 g / L, MgSO 4 ·7H 2 O 0.2 g / L, MnSO 4 ·H 2 O 0.01 g / L, NaCl 0.01 g / L, FeSO 4 ·7H 2 O 0.01 g / L; corn steep liquor 1 g / L.

[0052] In this experiment, corn stalks were taken as an example to illustrate the specific steps of selecting the dosage of polyethyleneimine modifier.

[0053] The addition amount of corn straw was 10 g / L (modified by 4 g / L polyethyleneimine and 1 g / L glu...

Embodiment 3

[0055] This example is to demonstrate the application of modified rice straw as an immobilized carrier in butanol fermentation.

[0056] Bacteria: Clostridium acetobutylicum Clostridium acetobutylicum AS1.135 (CGMCC 1.135)

[0057] Seed medium: Seed medium: yeast powder 3 g / L, peptone 5 g / L, soluble starch 10 g / L, ammonium acetate 2 g / L, NaCl 2 g / L, MgSO 4 3 g / L, KH 2 PO 4 1 g / L, K 2 HPO 4 1 g / L, FeSO 4 ·7H 2 O 0.1 g / L, pH 6.0;

[0058] Fermentation medium (P2): carbon source (glucose 60 g / L dissolved), K 2 HPO 4 0.5 g / L, KH 2 PO 4 0.5 g / L, CH 3 COONH 4 2.2 g / L, MgSO 4 ·7H 2 O 0.2 g / L, MnSO 4 ·H 2 O 0.01 g / L, NaCl 0.01 g / L, FeSO 4 ·7H 2 O 0.01 g / L; corn steep liquor 1 g / L.

[0059] In this experiment, rice straw was taken as an example to illustrate the specific steps of selecting the dosage of polyethyleneimine modifier.

[0060] The addition amount of rice straw was 10 g / L (modified by 4 g / L polyethyleneimine and 1 g / L glutaraldehyde, the ratio o...

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Abstract

The invention relates to a chemical modified fiber type immobilization carrier as well as a preparation method and an application thereof. The invention provides a method for preparing the chemical modified fiber type immobilization carrier. The method comprises the steps as follows: 1), the fiber type immobilization carrier is soaked in a polyethyleneimine solution, so that the carrier modified by polyethyleneimine is obtained, wherein, polyethyleneimine molecules are formed in the surface of the carrier modified by the polyethyleneimine; 2), the carrier modified by the polyethyleneimine is soaked in a glutaraldehyde solution, so that amino groups and aldehyde groups of glutaraldehyde in the polyethyleneimine molecules have reactions, and the chemical modified fiber type immobilization carrier is obtained. According to an embodiment of the chemical modified fiber type immobilization carrier, the chemical modified fiber type immobilization carrier can be taken as a cell immobilization carrier and can be filled to form an immobilization fiber bed, and is applied to single-batch fermentation and consecutive batch fermentation of biobutanol.

Description

technical field [0001] The present invention belongs to the field of biochemical technology, specifically, it relates to a chemically modified fiber immobilized carrier and its preparation method and application, more specifically, it relates to a method for preparing a chemically modified fiber immobilized carrier, and a chemically modified fiber immobilized carrier An immobilized carrier, a device for preparing butanol and a method for preparing butanol using the device. Background technique [0002] Affected by world oil resources, prices, environmental protection and global climate change, the development of biofuels has become an important measure for many countries to improve energy security, reduce greenhouse gas emissions, and address climate change. Bio-butanol is mainly produced by Clostridium through anaerobic fermentation. This method can effectively alleviate the two outstanding problems of energy shortage and environmental pollution. As a new type of liquid fue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/12C08H8/00C12M1/00C12P7/16C12R1/145
Inventor 姜岷孔祥平贺爱永吴昊陈佳楠尹春燕陈吴方陈攀
Owner NANJING UNIV OF TECH
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