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Method for producing high-functional-trisaccharide-content isomaltooligosaccharide by using immobilized cells

A technology of isomaltooligosaccharides and immobilized cells, applied in the direction of microorganism-based methods, biochemical equipment and methods, fixed on/in organic carriers, etc., to achieve reduced water consumption, high substrate conversion efficiency, and process simplified effect

Active Publication Date: 2015-07-15
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the production of isomaltooligosaccharide by immobilization technology is concentrated in the field of immobilized enzymes, but there is no research report on the co-production of isomaltooligosaccharide by immobilized cells and simulated moving bed chromatographic separation technology

Method used

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  • Method for producing high-functional-trisaccharide-content isomaltooligosaccharide by using immobilized cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for immobilized cells to produce isomaltooligosaccharide with high functional trisaccharide content, the steps are as follows:

[0042](1) The seed liquid containing Aspergillus niger (Aspergillus niger) BLB‐28 strain was filtered out 10% by mass percentage of water, and then mixed with sodium alginate with a mass concentration of 3% in a volume ratio of 1:1, Then evenly drip it into a calcium chloride solution with a mass concentration of 3% for fixation for 4 hours, take out the immobilized rubber beads, wash them with water, and immerse them in pH 5‐7, containing 0.5% calcium chloride and 0.1% The mixed solution of chitosan is then soaked for 6h, and then the immobilized cells are loaded into the column by 20% of the column volume to obtain an immobilized cell column;

[0043] (2) Use cornstarch for pulping, the pulping concentration is 20% (mass concentration), and adjust the pH to 5.5, add high-temperature α-amylase, the addition amount is 0.2L per ton of ...

Embodiment 2

[0048] A method for immobilized cells to produce isomaltooligosaccharide with high functional trisaccharide content, the steps are as follows:

[0049] (1) The seed liquid containing Aspergillus niger (Aspergillus niger) BLB-28 strain was filtered out 40% of the water by mass percentage, and then mixed with sodium alginate with a mass concentration of 3% in a volume ratio of 1:5, Then evenly drop it into a calcium chloride solution with a mass concentration of 3% and fix it for 12 hours, take out the immobilized rubber beads, wash them with water, and immerse them in pH 5‐7, with a mass concentration of 3% calcium chloride and a mass concentration of 2%. The mixed solution of chitosan is then soaked for 12 hours, and then the immobilized cells are loaded into the column by 80% of the column volume to obtain the immobilized cell column;

[0050] (2) Use cornstarch for pulping, the pulping concentration is 40% (mass concentration), and adjust the pH to 6.5, add high-temperature ...

Embodiment 3

[0055] A method for immobilized cells to produce isomaltooligosaccharide with high functional trisaccharide content, the steps are as follows:

[0056] (1) The seed solution containing Aspergillus niger (Aspergillus niger) BLB‐28 strain was filtered out with a mass percentage of 20% water, and then mixed with sodium alginate with a mass concentration of 3% in a volume ratio of 1:2, Then evenly drip it into a calcium chloride solution with a mass concentration of 3% and fix it for 6 hours, take out the immobilized rubber beads, wash them with water, and immerse them in pH 5-7, with a mass concentration of 2% calcium chloride and a mass concentration of 1%. The mixed solution of chitosan is then soaked for 12h, and then the immobilized cells are loaded into the column by 70% of the column volume to obtain an immobilized cell column;

[0057] (2) Use cornstarch for pulping, the pulping concentration is 32% (mass concentration), and adjust the pH to 6.3, add high-temperature α-amy...

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Abstract

The invention relates to a method for producing high-functional-trisaccharide-content isomaltooligosaccharide by using immobilized cells. The method comprises the following steps: liquefying and saccharifying the raw material starch to obtain malt syrup; and preparing the high-functional-trisaccharide-content isomaltooligosaccharide by combining an immobilized Aspergillus niger cell technique and a simulated moving bed chromatographic separation technique. After the saccharified liquid is subjected to glycoside transformation by the immobilized cells, the total amount of the three functional components (isomaltose IG2, panose P and isomaltotriose IG3) is up to higher than 60%, the transformation ratio of the immobilized cell substrate is higher than that of the immobilized enzyme, and the indexes of the product are much higher than those of like products at home and abroad; after the chromatographic separation, the total amount of the three functional components can reach higher than 90%; and meanwhile, the method greatly shortens the reaction time, and is convenient to operate and suitable for large-scale continuous production.

Description

technical field [0001] The invention relates to a method for immobilized cells to produce isomaltooligosaccharides with high functional trisaccharide content, in particular to a method for co-producing isomaltooligosaccharides with high functional trisaccharide content by using immobilized cells and simulated moving bed chromatographic separation technology. The method for maltose belongs to the technical field of starch sugar production. Background technique [0002] Isomaltooligosaccharide is the sugar with the largest yield and the most wide application among functional oligosaccharides. It cannot be utilized by harmful bacteria in the human large intestine, but it can effectively promote the proliferation of bifidobacteria, thereby promoting gastrointestinal peristalsis and improving The micro-ecological environment of the gastrointestinal tract improves the body's immunity, decomposes intestinal toxins and carcinogens, and isomaltooligosaccharide also has the function o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/18C12P19/14C12N11/10C12N11/04C12R1/685
Inventor 刘宗利王乃强贾慧慧薛雅莺李方华
Owner BAOLINGBAO BIOLOGY
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