Method and kit for dual fluorescence quantitative PCR detection of grass carp reovirus types I and II

Abstract

The invention discloses a method for dual fluorescence quantitative PCR detection of grass carp reovirus types I and II. The method can realize that the grass carp reovirus types I and II can be simultaneously detected in one to-be-detected sample, and the load levels of the two reovirus types can be quantified. The invention further provides a kit for dual fluorescence quantitative PCR detection of the grass carp reovirus types I and II. According to the detection method and the kit which are provided by the invention, the operation is simple and convenient, the specificity is strong, the detection sensitivity and accuracy rate are high and reliable, and a wide application prospect is achieved.

Description

technical field [0001] The invention relates to a dual fluorescent quantitative PCR detection kit, in particular to a dual fluorescent quantitative PCR detection kit for grass carp reovirus type I and type II, and further relates to a grass carp reovirus type I and type II Type of double fluorescent quantitative PCR detection method. Background technique [0002] Grass carp reovirus (GCRV) belongs to the genus Aquatic Reovirus, a new member of Reoviridae, and the first fish virus isolated from mainland China. The virus mainly causes haemorrhagic disease in freshwater cultured grass carp species in China, Vietnam, Myanmar and other Asian countries, and the mortality rate can be as high as 60%. The virus is widespread, harmful, high in mortality, and has a long onset season, which seriously threatens fishery production. Grass carp reovirus has a double-layer capsid, the average diameter of the virion is 60 nm to 70 nm, icosahedral symmetry, no envelope, and the genome consis...

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Problems solved by technology

[0004] There are many methods for detecting grass carp reovirus, each with its own advantages and disadvantages. Virus isolation, electron microscope observation, and nucleic acid band type analysis are still commonly used methods for detecting grass carp reovirus, but these methods are time-consuming and labor-intensive. Complicated, unable to quickly diagnose the virus, which is not conducive to the epidemiological investigation of grass carp haemorrhagic disease

Whole virus diagnosis involves virus culture, purification, concentration and other processes. Not only is the production process complicated, time-consuming, and costly, but it also has the potential to spread the virus, so it is not conducive to popularization and use.

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