On-line measurement method of biofilm photobioreactor

A technology of photobioreactor and measurement method, applied in the field of high-efficiency biofilm photobioreactor online measurement, can solve the problem of inhibition of hydrogen production metabolic pathway, low biofilm active biomass, increased substrate and metabolite transmission resistance and light Energy attenuation and other issues, to achieve the effect of increasing the hydrogen production rate

Active Publication Date: 2015-08-05
CHONGQING UNIV
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Problems solved by technology

When the thickness of the biofilm is thin, the biomass holding capacity in the reactor is low, and the hydrogen production capacity of the reactor is low
However, uncontrolled thicker biofilms did not produce more hydrogen than thin biofilms because thicker biofilms, while increasing bioholding, also increased substrate And the transmission resistance of metabolites and the attenuation of light energy, the active biomass in the biofilm is low, and the metabolic pathway of hydrogen production is inhibited
Therefore, both too high and too low biofilm thickness will limit or inhibit the hydrogen production capacity of the reactor

Method used

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  • On-line measurement method of biofilm photobioreactor
  • On-line measurement method of biofilm photobioreactor
  • On-line measurement method of biofilm photobioreactor

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[0059] 1. Strains and culture medium

[0060] The experiment used the Rhodoseudomonas palustris CQK-01 (abbreviated as PSB CQK-01) isolated, identified and domesticated by the Institute of Engineering Thermophysics of Chongqing University. The culture method of PSB CQK-01 mother liquor is: anaerobic 96h under the conditions of temperature, light conditions, and pH of 30℃, 590nm, 4000lx, 7.0. In view of the physiological characteristics of PSB CQK-01 strain, the medium composition and its ratio are as follows: ZnSO 4 ·7H 2 O(0.001g),(NH 4 ) 6 Mo 7 O 24 ·4H 2 O(0.001g), CaCl 2 (0.01g),MgSO 4 ·7H 2 O(0.2g), NaCl(0.2g), KH 2 PO 4 (0.554g), FeSO 4 ·7H 2 O(0.417g), K 2 HPO 4 ·3H 2 O(1.006g), CO(H 2 N) 2 (1.677g), C 5 H 8 NNaO 4 (0.5g), yeast extract (1.0g), C 6 H 12 O 6 ·H 2 O (10g), 1 mL of growth factor solution. The composition and ratio of growth factors are: biotin 1g / L, pyridoxine hydrochloride 1g / L, riboflavin 1g / L, thiamine hydrochloride 1g / L, and niacin 1g / L. The reagents use...

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Abstract

The invention discloses an online measurement method for a high-efficiency biofilm photobioreactor. The steps are as follows: the first stage is to pass the suspension of photosynthetic bacteria into the reactor in a closed loop manner, and the flow rate is controlled at 60-120 mL / h, the liquid phase temperature is 30 ℃, and the pH value is 7.0. After the photosynthetic bacteria suspension is filled with the reactor box, continue to run for 6-10 h, and then stand still for 24-36 h; the second stage is to adopt continuous flow culture In this way, the fresh medium with a pH of 7.0 is continuously fed into the reactor box, the flow rate is 60-120 mL / h, and the temperature is 30 °C; the waste liquid of the medium passes through the gas collection bottle and is directly discharged from the reactor system. The invention adopts a sensor for online measurement of biomass concentration, pH and hydrogen concentration; the biofilm thickness and pH in the reactor can be regulated according to the real-time measurement results of the sensor; the hydrogen production rate of the regulated biofilm reactor is improved.

Description

Technical field [0001] The invention relates to an online measurement method for a biofilm reactor, in particular to an online measurement method for a high-efficiency biofilm photobioreactor. Background technique [0002] The price of fossil energy continues to rise, and energy shortages have increasingly become the primary problem that plagues social development. At the same time, the excessive exploitation of fossil fuels has caused severe damage to the natural environment. Actively develop environmentally friendly and economical renewable energy sources, especially the vigorous development of biomass energy, which can effectively alleviate the pressure of energy shortage and become a major strategy related to China's sustainable development. demand. [0003] Biomass energy resources include energy plants and crops, wood, forest and garden industrial wastes, animal manure, etc. Biomass conversion platforms mainly include thermochemical conversion method platforms and biologica...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/25G01B11/06G01K11/32G01N27/00G01K11/3206
Inventor 朱恂钟年丙廖强陈蓉王宏叶丁丁丁玉栋李俊
Owner CHONGQING UNIV
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