Applications of urine molecule
A molecular and urine technology, applied in the application field of urine molecules, can solve the problems of insufficient early diagnosis, complicated detection methods, and insufficient specificity of detection, and achieve the effect of convenient sampling and low detection price
Inactive Publication Date: 2014-01-01
刘红莉
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Problems solved by technology
At present, the detection of serum AFP (Alpha Fetal Protein, alpha-fetoprotein) is still the main marker for laboratory screening and diagnosis of liver cancer. Although its positive rate for liver cancer diagnosis can reach 70-90%, there are still many defects: 1 .Diagnosis is not early enough
2. The test is not specific enough, there are some false positives in the test results, and there are many influencing factors
3. The detection method is relatively complicated, and the commonly used radioimmunoassay has radioactive contamination
But the fact is that there are almost no patients diagnosed in the early stage, and the liver cancer is often found in the late stage, for which various treatment methods may not be helpful
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[0074] The levels of LRG1 and CD13 can be detected by many qualitative and quantitative methods, such as gold standard method, absorbance method, protein concentration assay (such as DC protein assay, BCA protein assay and Lowry protein assay), electrophoretic separation ( Such as SDS-PAGE gel purification), Western blot (such as western blot), chromatographic analysis (such as molecular size chromatography, ion exchange chromatography and affinity column chromatography), precipitation, ultracentrifugation, enzyme-linked immunoassay (ELISA method) and other common techniques. In addition, the quantitative analysis of LRG1 and CD13 can also be carried out with the detection kit.
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The invention discloses applications of a urine molecule, which can be used for detection of tumor canceration and indicates whether an individual or a person tested has a cancer or not or whether the individual or the person tested has the possibility to have a cancer or not. Through carrying out different molecule screening of tested tumor urine, the level contents of leucine-rich-2-glycoprotein-1 and aminopeptidase N in the tested tumor urine sample can be detected, testified, and analyzed, wherein the different molecule expression of leucine-rich-2-glycoprotein-1 is in a positive correlation with tumor canceration, and the different molecule expression of aminopeptidase N is in a positive correlation with tumor canceration malignancy degree. The two tumor-related molecule markers provide novel and convenient lab detection indicators for screening and diagnosing whether an individual has the possibility to have a cancer or an individual has already had a cancer. Compared to the conventional tumor detection methods, which need to draw blood or carry out tissue puncture, the tumor-related molecule detection method provided by the invention has the advantages of non-invasiveness and convenient sampling, and is especially suitable for large-scale screening of tumor high-risk group and detections in basic medical institutions.
Description
technical field [0001] The invention belongs to the field of medical diagnosis, in particular to the application of urine molecules for early detection of tumor canceration. Background technique [0002] Cancer is a disease caused by the abnormal mechanism of cell growth and proliferation. In addition to growing out of control, malignant cells also lose the original shape and function of the original cells to varying degrees. They can also invade surrounding tissues and even pass through the circulatory system Or the lymphatic system is transferred to other parts of the body, leading to malignant outcomes and even death in patients. At present, the cause of cancer has not been fully elucidated, so there is a lack of effective preventive measures. If cancer can be diagnosed at an early stage, the success rate of its treatment will be greatly improved. [0003] Generally speaking, the diagnosis of cancer mainly depends on the patient's clinical manifestations, laboratory tes...
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Inventor 刘红莉周爱民曾春
Owner 刘红莉
