Method of preparing flaccid anemone saponins W1 and W3 from rhizome of flaccid anemone
A technology of kunain and black medicinal materials, which is applied in the field of preparation of kusaponin W1 and W3, which can solve the problems of unfavorable safety management, high toxicity, and serious environmental pollution, and achieve the effects of reducing production costs, reducing health threats, and reducing pollution
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Embodiment 1
[0040] 1) Take 1 kg of dried Diwu raw material and add it to the heating vessel. The raw material of Diwu medicinal material is generally in powder form.
[0041] 2) Pour 10L (10kg) of water into the heating vessel, heat and decoct for 1 hour, filter out the decoction, and keep the residue in the heating vessel.
[0042] 3) Repeat step 2) once, and combine the decoction filtered out each time.
[0043] 4) First, put the combined decoction on a D-101 macroporous resin column;
[0044] Then, carry out gradient elution with water, 10% ethanol, 50% ethanol and 95% ethanol solution in sequence, and achieve the purpose of removing impurities and cleaning through the elution treatment of water, 10% ethanol and 95% ethanol solution, and collect 50% ethanol wash The eluate after de-treatment,
[0045] Finally, the collected eluate was concentrated and dried under reduced pressure to obtain 62 g of the total saponins extract.
[0046] 5) Firstly, dissolve the obtained Diwu saponin ex...
Embodiment 2
[0056] 1) Take 1 kg of dried Diwu raw material and add it to the heating vessel. The raw material of Diwu medicinal material is generally in powder form.
[0057] 2) Pour 8L (8kg) of water into the heating vessel, heat and decoct for 3 hours, filter out the decoction, and keep the residue in the heating vessel.
[0058] 3) Repeat step 2) twice, and combine the decoction filtered out each time.
[0059] 4) First, put the combined decoction on the AB-8 macroporous resin column;
[0060] Then, carry out gradient elution with water, 25% ethanol, 80% ethanol and 95% ethanol solution in sequence, and achieve the purpose of removing impurities and cleaning through the elution treatment of water, 25% ethanol and 95% ethanol solution, and collect 80% ethanol wash The eluate after de-treatment,
[0061] Finally, the collected eluate was concentrated and dried under reduced pressure to obtain 58 g of the total saponins extract.
[0062] 5) Firstly, dissolve the obtained Diwu saponin e...
Embodiment 3
[0072] 1) Take 1 kg of dried Diwu raw material and add it to the heating vessel. The raw material of Diwu medicinal material is generally in powder form.
[0073] 2) Pour 5L (5kg) of water into the heating vessel, heat and decoct for 2 hours, filter out the decoction, and keep the residue in the heating vessel.
[0074] 3) Repeat step 2) twice, and combine the decoction filtered out each time.
[0075] 4) First, put the combined decoction on the AB-8 macroporous resin column;
[0076] Then, carry out gradient elution with water, 35% ethanol, 75% ethanol and 95% ethanol solution in sequence, and achieve the purpose of removing impurities and cleaning through the elution treatment of water, 35% ethanol and 95% ethanol solution, and collect 75% ethanol wash The eluate after de-treatment,
[0077] Finally, the collected eluate was concentrated and dried under reduced pressure to obtain 60 g of the total saponins extract.
[0078] 5) Firstly, dissolve the obtained Diwu saponin e...
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