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Method for cultivating microorganisms

A microbial cultivation and microbial technology, applied in the field of microbial cultivation, can solve the problems of increasing energy consumption in the microbial cultivation process, inability to scale up the mode of the microbial incubator, and limiting the application of the microbial incubator, so as to improve the efficiency of material and energy utilization, design and Flexible assembly method, increase the effect of gas-liquid agitation frequency

Inactive Publication Date: 2014-01-08
钟琦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantages of these three methods are: 1. all will increase the energy consumption of the microbial culture process, thereby increasing the production cost; 2. the mode of the microbial incubator cannot be enlarged to the scale of industrial production; ④ For microalgae cultivation, the existing technology can only provide carbon dioxide gas and light energy to a small number of microalgae cells for photosynthesis at the same time, which reduces the cultivation efficiency

Method used

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  • Method for cultivating microorganisms
  • Method for cultivating microorganisms
  • Method for cultivating microorganisms

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Embodiment Construction

[0030] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0031] The culture method of microorganism of the present invention comprises the following steps:

[0032] The microorganisms are introduced into the incubator with the culture medium, and the gas is introduced for cultivation;

[0033] The incubator includes a container and a bubble guide assembly arranged in the container, the bubble guide assembly includes at least two zigzag guide members; the zigzag guide member includes at least two guide plates connected up and down in sequence in a vertical direction, and the guide plates are inclined relative to the horizontal plane Set up, and any adjacent guide plates in the same zigzag guide member have opposite inclination directions relative to the horizontal plane, and a zigzag rising channel is formed between any adjacent zigzag guide members; any guide plate of the zigzag guide member and the ad...

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Abstract

The invention discloses a method for cultivating microorganisms. Microorganisms are introduced into a culture vessel with culture solution, and gas is introduced for cultivating. The culture vessel comprises a bubble guide component, a zigzag guide member and a guide plate of the bubble guide component provide bubbles with a zigzag upward march path, the introduced gas forms bubbles through a bubble generator, the bubbles enter from the bottom of the bubble guide component and climb upwards circuitously along the lower surface of the guide plate, and the bubbles and the culture solution with microorganisms change substance in climbing. The method for cultivating the microorganisms adopts the culture vessel comprising the bubble guide component of special structure, so that the contact area and the contact time of the bubbles and the culture solution in unit volume are effectively increased, the culture efficiency is improved, and the culture cost is reduced; and the design and assembly manner of the bubble guide component are flexible, the bubble guide component has strong adaptability, so that the method for cultivating microorganisms has good industrial application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a microorganism culture method. Background technique [0002] In the process of microbial cultivation, it is usually necessary to transfer gaseous nutrients from the atmosphere or artificial gas storage containers to the culture solution of microorganisms in the form of bubbles, especially photosynthetic microorganisms such as algae, which need to be in the presence of solar radiation and rich sources such as carbon dioxide. In the case of carbon-containing gas, growth and photosynthesis in a suitable nutrient culture medium, thus increasing the supply of gaseous nutrients in the microbial culture medium is beneficial to the growth of microorganisms. At present, there are three ways to increase the supply of gaseous nutrients: the first is to increase the gas transmission rate, and more gas is forced into the microbial culture environment within a certain period of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12R1/01
Inventor 钟琦
Owner 钟琦
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