Molecular marker InDe1587 for detecting rice blast resistant gene Pigm(t) of rice #4

Abstract

The invention discloses a molecular marker InDe1587 for detecting a rice blast resistant gene Pigm(t) of rice #4. The molecular marker InDe1587 disclosed by the invention is positioned at the sixth chromosome 10484756-10484985bp, is spaced from a positioning range 10367733-1042226bp of the rice blast resistant gene Pigm(t) at 65.2 kg and is closely linked with the rice blast resistant gene Pigm(t); furthermore, the marker InDe1587 is high in polymorphism and the polymorphism can be realized between 87.2 percent of receptors and the rice #4 of a donor; PCR (polymerase chain reaction) products of the InDe1587 are greatly different; the marker gene type of a material can be quickly detected. The material to be detected can be detected through the molecular marker, so that whether the material carries the rice blast resistant gene Pigm(t) can be judged.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker InDel587 for detecting the rice blast resistance gene Pigm(t) of Gumei No. 4. Background technique [0002] Most of the molecular markers published in the rice blast resistance gene Pigm(t) literature (TheorAppl Genet, 2006, 113:705-713) are CAPS markers, which are not suitable for molecular marker-assisted selection, and SSR and InDel markers are mainly used for genetic mapping Yes, there are disadvantages of low polymorphism rate and small differences in breeding. Traditional rice disease resistance breeding is phenotypic selection of plants through resistance identification, which takes a long time and is easily limited by environmental conditions. The identification results are prone to errors and the selection efficiency is low. [0003] Rice blast is one of the three major diseases that endanger rice production in my country, and molecular marker-assisted se...

AI Technical Summary

Problems solved by technology

Gumei 4 has broad-spectrum rice blast resistance, and Pigm(t) is located in the range of about 70kb between molecular markers C5483 and C0428 on chromosome 6. The molecular markers published in the literature for genetic mapping are not suitable for marker assisted breeding

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