New pure white hypsizigus marmoreus strain
A technology of white shimeji mushrooms and strains, applied in fungal products, botanical equipment and methods, horticulture, etc., can solve the problems of poor appearance uniformity, long cultivation period, and high production cost, and achieve reduced production cost, high yield, The effect of improving the appearance uniformity
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Embodiment 1
[0025] Example 1 Obtaining Finc-W-90 by hybridization
[0026] The breeding process of the present invention's pure white mushroom strain Finc-W-90 comprises the following steps:
[0027] 1) Select parental strains: select TNN-11 and H-W as parents, the fruiting bodies of these two parents can be purchased in the market, and are also preserved in the strain collection center of Shanghai Academy of Agricultural Sciences.
[0028] 2) Preparation of culture medium
[0029] PDA medium: use 200g of potatoes, 20g of glucose and 20g of agar per 1L of medium.
[0030] Original seed culture medium: 65% of sawdust (percentage by weight, the same below), 18% of rice bran, 7% of bran, 10% of corn flour, and the water content is 60% to 62%.
[0031] Cultivated medium: 40% corn cob, 24% soybean hulls, 14% rice bran, 17% bran, 5% corn flour, and the water content is 64% to 66%.
[0032] 3) Obtain 800 heterozygotes by conventional methods.
[0033] 4) Cultivation screening: The obtained h...
Embodiment 2
[0036] Embodiment 2 bacterial age test
[0037] The present embodiment compares the cultivation period (i.e. the bacterial age, the same below), the cultivation period and the unit yield of the pure white shimeji mushroom strain Finc-W-90 of the present invention with the most widely planted H-W in the existing pure white shimeji mushroom, To illustrate the advantages and characteristics of the pure white mushroom strain Finc-W-90 of the present invention.
[0038] The cultivar culture medium used in this embodiment is: 65% sawdust, 18% rice bran, 7% bran, 10% corn flour, water is added to make the water content in the medium reach 60%-62%.
[0039] Taking H-W as a control, set 9 bacterial ages from 70d (that is, days, the same below) to 110d, and every increase of 5d is a bacterial age, and each bacterial age is 80 bottles. Cultivation and cultivation adopt conventional shimeji mushroom cultivation and cultivation parameters, the volume of the cultivation bottle is 850cc, an...
Embodiment 3
[0043] Embodiment 3 Cultivation comparative test
[0044] In this example, the uniformity of bottle yield, cap, stipe length and fresh weight of single fruiting body of two strains of Finc-W-90 and H-W was studied. Taking Finc-W-90 and H-W at the 85th day age in Example 2 as the research objects, 40 cultivation bottles were randomly selected as samples from different strains for statistical analysis. The results are shown in Table 2-Table 5.
[0045] Wherein, coefficient of variation (%)=standard deviation / mean value×100% (the same below)
[0046] According to Table 2, the coefficient of variation of the yield per unit area of Finc-W-90 is 7.4%, and the coefficient of variation of yield per unit area of H-W is 15.5%. It can be seen that the relative variability of yield per unit area of Finc-W-90 is small, indicating that the yield of Finc-W-90 mushrooms Yields are more even.
[0047] Table 2 Uniformity of yield per unit area of different strains of Shijiji mushroom
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