Tissue culture medium of sealwort roots and stems and in-vitro regeneration method

Abstract

The invention discloses a tissue culture medium of sealwort roots and stems and an in-vitro regeneration method of the tissue culture medium. The tissue culture medium comprises an inducing culture medium, a proliferation culture medium and a rooting culture medium. The method using the tissue culture medium of the sealwort roots and stems for carrying out in-vitro regeneration on the sealwort roots and stems comprises the three steps of induction of sprouts, proliferation culture of the sprouts and rooting culture. The tissue culture medium and the propagation method have the beneficial effects that a system of in-vitro regeneration of the sealwort roots and stems is established by utilizing a tissue culture technology, and the inducing culture medium, the proliferation culture medium and the rooting culture medium in the method are selected and optimized; and the tissue culture medium has the advantages of high sprout induction rate, high proliferation coefficient, high rooting rate, strong sprouts and seedlings, normal leaf shape and color and the like, and can meet the need of the market for the sealwort roots and stems.

Description

Technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a tissue culture medium of Polygonatum rhizome and a method for in vitro regeneration. Background technique [0002] Polygonatum (Polygonatum sibiricum Red.) is the general name for the rhizomes of various plants in the genus Polygonatum Mill. in the Liliaceae family. It is a perennial herbaceous medicinal plant whose rhizome is a traditional Chinese medicine. The Pharmacopoeia of the People’s Republic of China in 2005 (Part One) stipulates that Polygonatum sibiricum Delar.ex Redoute, P.cyrtonema Hua and P.kingianum Coll.et Hemsl. Source of native medicine. Polygonatum contains polysaccharides, amino acids, anthraquinone compounds and other ingredients, which have the effects of antibacterial, lowering blood pressure, anti-aging and curing rheumatism. [0003] As the medicinal value and health care value of Polygonatum are more and more recognized by people, the demand for ...

AI Technical Summary

Problems solved by technology

The people such as Li Shi have carried out research to the wild change home species cultivation of Polygonatum, and some places also begin to artificially plant Polygonatum, but because the multi-root propagation that adopts in production, its reproduction coefficient is low, and kind of rhizome consumption is big, both uneconomical, and It limits the yield potential of Rhizoma Polygonatum, which is inconvenient for planting management and promotion, and the long-term vegetative propagation by splitting roots can easily cause the degradation of Rhizoma Polygonatum species, so the problem of Rhizoma Polygonatum seedlings has become a bottleneck for large-scale artificial planting

The use of plant tissue culture technology to establish the in vitro regeneration system of Polygonatum is an effective way to produce high-quality Polygonatum seedlings, but there is no relevant report on the in vitro regeneration of Polygonatum through tissue culture

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