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Living molecular detection method for precocious puberty individual of Eriocheir sinensis

A kind of Chinese mitten crab, individual technology, applied in the field of genetic engineering

Inactive Publication Date: 2014-02-05
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] At present, several shrimp and crab EcR genes have been cloned, including: Marsupenaeus japonicas, Fenneropenaeus chinensis, Crangon crangon, etc., but the EcR gene of Chinese mitten crab (here named: EsEcR) has not been reported

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  • Living molecular detection method for precocious puberty individual of Eriocheir sinensis

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Embodiment Construction

[0036] Below in conjunction with embodiment, further set forth the present invention.

[0037]Eriocheir sinensis in the present invention is provided by Wuxi Taihu Lake fishermen, reagents can be purchased from the market, reagents such as RNA purification are purchased from Promega Company of the United States, etc., 3'RACE and 5'RACE kits are purchased from Bao Biological (Dalian) Co., Ltd. ( Takara).

[0038] The method for amplifying the EsEcR gene of Chinese mitten crab comprises the following steps:

[0039] (1) Total RNA extraction:

[0040] Take 100 mg of Chinese mitten crab tissue and grind it with liquid nitrogen in a mortar, and extract total RNA by Trizol method. Trizol reagent is from Invitrogen Company, and the extraction method is according to the instruction manual.

[0041] (2) Cloning of the full-length cDNA sequence of EsEcR gene of Chinese mitten crab:

[0042] A 217bp EcR gene homologous sequence (GenBank accession number: JR736395.1) was screened from ...

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Abstract

The invention discloses a living molecular detection method for precocious puberty individual of Eriocheir sinensis. Full-length cDNA sequence of EsEcR gene is cloned from Eriocheir sinensis tissue by the use of primer groups provided by the invention. Based on the gene sequence, the invention provides a living molecular detection method for precocious puberty individual of Eriocheir sinensis. The invention not only provides a living molecular detection method for precocious puberty individual of Eriocheir sinensis, but also provides an important testing method for early detection of Eriocheir sinensis precocious puberty individual.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a method for cloning EsEcR gene of Chinese mitten crab and a method for detecting live molecules of precocious individuals of Chinese mitten crab based on the EsEcR gene. Background technique [0002] The Chinese mitten crab (Eriocheir sinensis) belongs to Arthropoda, Crustacea, Decapod, Reptilia, Brachycephalus, Square Crabidae, Archipodinae, Eriocheir genus, commonly known as river crab, widely distributed in southeastern my country In coastal brackish water or fresh water, its meat is delicious and unique in flavor, and river crab is the freshwater crab with the largest farming output in China. As a crustacean, the growth of the river crab is reflected in the form of molting. Molting, the process of shedding the old exoskeleton and growing a new one, is the most prominent physiological feature of crustaceans. The sexual maturity of river crabs has an inhibitory ...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/10C12Q1/68C12N15/11
Inventor 沈怀舜周鑫
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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