Preparation method of raja porosa extract as well as application of raja porosa extract in alcoholic liver protection medicament

Abstract

The invention discloses a preparation method of a raja porosa extract, and an application of the raja porosa extract in preparing an alcoholic liver protection medicament. The preparation method is characterized by comprising the following steps: boiling fresh raja porosa, subsequently peeling off the meat, washing the soft bone in clean water, crushing the soft bone, adding a NaOH solution according to a material-liquid mass ratio of 1:(1-1.5), stirring and extracting for 4 hours, subsequently adjusting the pH value to be 6 to 7 by using HCl, filtering, adding NaOH into the filtrate to adjust the PH value to be 8.5-9.0, adding compound protease to hydrolyze for 4 hours according to a mass ratio of the material liquid to the compound protease of 1,000:1, heating the hydrolysate for 30 minutes to be 80 DEG C, adding HCl to adjust the pH value to be 2, standing, performing suction filtration on the solution at the upper layer by using kieselguhr, adding NaOH to adjust the pH value to be 10.5, decoloring by using hydrogen peroxide, subsequently filtering, adding HCl into the filtrate to adjust the pH value to be 7, carrying out reduced-pressure concentration on the filtrate to be a small volume, precipitating and drying so as to obtain the raja porosa extract. The raja porosa extract can be used as an alcoholic liver injury protection agent as the activity of an antioxidant enzyme is improved and the cleaning capability of an organism to a peroxidatic reaction product is improved.

Description

technical field [0001] The invention relates to a preparation method of the ray extract and its application in the preparation of protective drugs against alcoholic liver damage. Background technique [0002] At present, alcoholism and addiction have become a global problem. Long-term heavy drinking can cause damage to multiple organs throughout the body, especially to the liver. Chronic alcoholism can lead to symptoms such as alcoholic fatty liver, alcoholic hepatitis, alcoholic cirrhosis, fibrosis, and in severe cases, canceration. At present, for the treatment of alcoholic liver poisoning, many methods include amino acid and glucose infusion, multivitamins, maintenance of water, electrolyte and acid-base balance, etc. In recent years, many researchers have tried to find a method that can effectively prevent and protect the liver from Medicines that lessen the damaging effects of alcohol on the liver. [0003] Kong ray (Raja porosa Günther), commonly known as Laoban fish...

AI Technical Summary

Problems solved by technology

The preparation method of the ray extract and its application in the preparation of alcoholic liver injury protection drugs have not been reported so far