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Kit for detecting procalcitonin

A technology of procalcitonin and reagent kit, which is applied in the field of immunoassay, can solve the problems of limited popularization, need, and inability to be widely used in clinical diagnosis and scientific research, and achieves no radioactive pollution, convenient use, and simple pretreatment process reliable effect

Inactive Publication Date: 2014-02-19
BEIJING LEADMAN BIOCHEM
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  • Claims
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Problems solved by technology

However, most of the chemiluminescent immunoassay kits in the prior art are imported closed automatic chemiluminescent detection systems, which require expensive automatic chemiluminescent detectors, which limits the promotion and use, and cannot be widely used in clinical diagnosis and treatment. scientific research

Method used

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  • Kit for detecting procalcitonin
  • Kit for detecting procalcitonin
  • Kit for detecting procalcitonin

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Embodiment Construction

[0023] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with specific embodiments and with reference to the accompanying drawings.

[0024] The kit for detecting procalcitonin provided by the present invention comprises magnetic particles coated with anti-fluorescein isothiocyanate polyclonal antibody, procalcitonin monoclonal antibody labeled with fluorescein isothiocyanate, alkaline phosphatase labeling Procalcitonin Monoclonal Antibody and Alkaline Phosphatase Catalytic Luminescence Chemiluminescent Substrate Solution.

[0025] Specifically, the kit for detecting procalcitonin can be prepared through the following steps:

[0026] 1. Preparation of PCT calibrator

[0027] Use pH=7.5, 0.1M Tris-HCl buffer to dilute PCT pure product into 6 levels of freeze-dried calibrator, and the target concentrations after reconstitution with pure water are 0, 0.25...

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Abstract

The invention discloses a kit for detecting procalcitonin. The kit comprises magnetic particles enveloped by a polyclonal antibody for resisting fluorescein isothiocyanate, a procalcitonin monoclonal antibody labeled by fluorescein isothiocyanate, a procalcitonin monoclonal antibody labeled by alkaline phosphatase, and chemiluminescent substrate liquid which is catalyzed by alkaline phosphatase to emit light. The kit for detecting procalcitonin, which is disclosed by the invention, adopts a double-antibody sandwich reaction mode and effectively makes use of a theory of combining a chemiluminescent detection technology and a magnetic particle immune separation technology to quantitatively measure the PCT (procalcitonin) content of a serum or plasma sample of a human body, so that the sensitivity of detection is guaranteed; furthermore, all indexes can reach the analysis level of the same type of imported kits.

Description

technical field [0001] The invention relates to the field of immune analysis, in particular to a kit for detecting procalcitonin. Background technique [0002] Procalcitonin (abbreviated as PCT) is a precursor substance of calcitonin (abbreviated as CT) without hormone activity. It comes from a single-copy gene located on chromosome 11 (11p15, 4). PCT is composed of 116 amino acids, A glycoprotein with a molecular weight of 13KD. PCT is composed of N-terminal-calcitonin-C-terminal three parts, which will not be degraded into calcitonin and will not be affected by hormone levels in the body. The half-life of PCT is 25-30 hours, which is one of the better indicators for detecting the inflammatory response caused by bacterial infection. Clinical data show that when the PCT concentration is higher than 0.1ng / mL, it indicates that there is a clinically relevant bacterial infection, and antibiotics are needed for treatment; when the PCT concentration is >0.5ng / mL, it is neces...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/577
CPCG01N33/74G01N33/533G01N33/577G01N2333/585
Inventor 不公告发明人
Owner BEIJING LEADMAN BIOCHEM
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