Membrane-separation-type culture device, membrane-separation-type culture kit, stem cell separation method using same, and separation membrane

Abstract

A membrane-separation-type culture device (1) which comprises an upper structure (10) that comprises a vessel involving, at at least a part thereof, a membrane (12) having pores (121) for allowing stem cells to pass therethrough and a lower structure (13) that comprises a vessel for holding a fluid in such an amount that the membrane in the upper structure can be immersed in the fluid; a membrane-separation-type culture kit which comprises the membrane-separation-type culture device (1) and a cell migration factor; a stem cell separation method which comprises a step of seeding cells or tissues of interest on the membrane (12) in the upper structure (10), a step of filling a culture medium containing a cell migration factor in the lower structure (13), and a step of bringing the membrane (12) in the upper structure (10) into contact with the culture medium in the lower structure (13); and a separation membrane which comprises a base membrane comprising a hydrophobic polymer and a functional layer comprising at least one hydrophilic polymer selected from a vinyl-pyrrolidone-type polymer, a polyethylene-glycol-type polymer and a vinyl-alcohol-type polymer which is bound to the surface of the base membrane via a covalent bond, wherein the weight of the hydrophilic polymer that constitutes the functional layer is 1.5 to 35 wt% relative to the total weight of the separation membrane.

Description

technical field The present invention relates to a membrane sorting culture device for sorting stem cells and dental pulp stem cells of any biological species, a membrane sorting culture kit, and a stem cell sorting method using the same. In particular, the present invention relates to a membrane sorting culture device for sorting dental pulp stem cells or mesenchymal stem cells filled in root canals for pulp regeneration, a membrane sorting culture kit, and a stem cell sorting method using the same. The present invention also relates to a separation membrane, a surface modification method, and the production of a separation membrane using the same, which provides a method for hydrophilizing a membrane composed of a hydrophobic polymer to suppress adhesion to the membrane when cells are permeated and separated without impairing the separation performance. separation membrane. Therefore, the present invention is applicable to the field of cell separation and purification center...

AI Technical Summary

Problems solved by technology

That is, a separation membrane module that satisfies blood compatibility with a high-performance separation membrane has not yet been established.

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