Extraction of Sodium Chondroitin Sulfate by Alkaline Hydrolysis-Enzyme Hydrolysis Combined with Flocculation and Precipitation

A technology of chondroitin sulfate sodium and enzymatic hydrolysis, which is applied in the field of biochemical pharmaceuticals, and can solve problems such as low extraction efficiency, cumbersome operation, and difficult purification

Active Publication Date: 2016-04-13
QINGDAO JIULONG BIO PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional extraction process adopts alkaline hydrolysis, enzymatic hydrolysis, salt hydrolysis and ion exchange resin or a combination thereof for extraction. The extraction efficiency is low and the operation is cumbersome. certain problem

Method used

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Examples

Experimental program
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Embodiment Construction

[0019] The following examples illustrate the invention in detail:

[0020] (1) Cartilage soaking: add 100Kg pig nose bone dry bone into the reaction tank and completely immerse it in water, after soaking for 4 hours, drain the soaking water;

[0021] (2) Alkaline hydrolysis: Add 250L of 1mol / L lye, stir and extract for 4 hours, filter with 100 mesh nylon mesh;

[0022] (3) Enzymolysis: After adjusting the pH to 8.5, heat up to 45°C, add 1Kg of trypsin, add 3Kg of sodium chloride, and keep warm for 4 hours at 45-50°C;

[0023] (4) Rapid temperature rise: heat up to 80°C, let stand for 20 minutes, and filter with a filter bag;

[0024] (5) add 95% ethanol 80L in filtrate;

[0025] (6) Flocculation and sedimentation: control the temperature at 45°C, adjust pH=11, add 1.2Kg of flocculation and sedimentation agent, siphon the supernatant after standing for stratification, centrifuge the sediment, merge the mother liquor into the supernatant, and discard the centrifuged residue; ...

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Abstract

The present invention relates to a sodium chondroitin sulfate extraction method, wherein sodium chondroitin sulfate is a mucopolysaccharide substance extracted from swine nasal bone and other animal cartilages. The traditional process method adopts alkaline hydrolysis, enzymolysis, salt hydrolysis, an ion exchange resin or a combination method thereof to extract, and the extraction solution contains a large amount of proteins, nucleic acids and other impurities so as to cause a certain difficult problem to further purification. According to the invention, the alkaline hydrolysis-enzymolysis method and the flocculation precipitation method are combined to remove impurities remained in the sodium chondroitin sulfate extraction solution, such that the process is reasonable, the removing efficiency is high, the subsequent refinement working hour is shortened, the product yield is improved, and the drug meets standards of the Chinese pharmacopoeia (2000 edition).

Description

technical field [0001] The field of the invention belongs to the field of biochemical pharmacy, and relates to a method for extracting sodium chondroitin sulfate, in particular to a production process for sodium chondroitin sulfate. Background technique [0002] Sodium chondroitin sulfate is an acidic mucopolysaccharide extracted from animal cartilage tissue, which belongs to glycosaminoglycan. Clinically, it is mainly used for lipid-regulating drugs, arthritis, eye drops, etc. Sodium chondroitin sulfate is an important biochemical drug on the market at present, and it is recorded in "Chinese Pharmacopoeia (2010 Edition)". In recent years, the trend of consumption of sodium chondroitin sulfate as a health product has gradually increased, making its application fields more and more extensive. [0003] The traditional extraction process adopts alkaline hydrolysis, enzymatic hydrolysis, salt hydrolysis and ion exchange resin or a combination thereof for extraction. The extrac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/0069
Inventor 刘乃山夏衬来迟培升陆玉梅
Owner QINGDAO JIULONG BIO PHARMA
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