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High-throughput nucleic acid continual amplification apparatus and method

A nucleic acid amplification device and continuous technology, applied in the field of PCR amplification reaction devices, can solve the problems of limited amplification, cumbersome PCR operation steps, and inability to operate continuously, and achieve the effect of improving work efficiency

Inactive Publication Date: 2014-02-26
杨锦宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problems in the prior art that the PCR amplification operation steps are cumbersome, the amplification amount is limited, and cannot be operated continuously, and a high-throughput continuous PCR amplification method is provided, which adopts the following technology Program:

Method used

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  • High-throughput nucleic acid continual amplification apparatus and method
  • High-throughput nucleic acid continual amplification apparatus and method

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Effect test

Embodiment 1

[0036] The temperature and time changes in the ordinary PCR amplification process are as follows: figure 1 shown. Divided into denaturation, annealing, extension process, each process has its own time and temperature.

[0037] The core structural module of the PCR amplification device provided by the invention is as figure 2 As shown, it is composed of three temperature control regions 21, which are arranged in sequence and connected end to end to form a closed ring structure. The temperature control regions respectively perform the steps of denaturation, annealing, and extension. The temperature in these areas can be set to the required reaction temperature. The capillary 11 is wound on the outside of the ring structure formed by the temperature control area 21. The capillary inlet 16 is arranged in the temperature control area where the denaturation reaction is performed, and the capillary outlet 17 is arranged in the area where the extension is performed. The temperatur...

Embodiment 2

[0043] The above-mentioned device can be as Figure 6 and Figure 7 This is installed into an all-in-one machine, which includes a display screen 1, a sample collection place cover plate 2, an upper cover 3, a lift switch 4, a sample adding place cover plate 5, and original sample storage tanks 6 and other parts.

[0044] Internal amplification system core components such as Figure 8 , Figure 9 and Figure 10 As shown, it includes a bracket 7, a pump 8, a detection window 12 (the fluorescence detector is installed here), and the temperature control unit 13 is arranged in a circle, and the middle of the circle is a temperature zone adjustment motor 9, and the motor is provided with 3 A group of disks 14, the disks 14 can be rotated separately, the disks 14 are respectively provided with brushes 15, the brushes 15 are connected with the temperature control unit 13, and the number of heating or cooling modules connected can be controlled by the rotation of the brushes 15. A...

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Abstract

The invention provides a high-throughput nucleic acid continual amplification apparatus and a method, and belongs to the technical field of biochemical apparatuses. The amplification method comprises the following steps: enabling a PCR reaction material to flow along a capillary tube, regulating the temperature of the reaction material by multiple temperature control areas arranged at the exterior of the capillary tube, and successively performing denaturation, annealing, extending on the PCR reaction material to finish the PCR amplification reaction. The amplification apparatus comprises three temperature control areas arranged in order, the temperature control areas are connected end to end and form an closed structure, the capillary tube is arranged in the temperature control areas and twines around the closed structure of the temperature control areas, the inlet of the capillary tube is arranged above the temperature control area for executing the denaturation reaction, the outlet of the capillary tube is arranged above the temperature control area for executing the extending reaction, and the inlet of the capillary tube is connected with a conveying device. The PCR amplification method and the apparatus help to realize continual amplification on a large batch of reaction systems, and help to realize simultaneous amplification and detection on multiple reaction systems or multiple samples.

Description

technical field [0001] The invention provides a device for PCR amplification reaction, which belongs to the technical field of biochemical equipment. Background technique [0002] Semi-conservative DNA replication is an important way of biological evolution and generation. The double-stranded DNA can be denatured and unwound into a single strand under the action of various enzymes. With the participation of DNA polymerase, it can be copied into the same two molecular molecules according to the principle of complementary base pairing. DNA can also be denatured and melted at high temperature, and can be annealed to become double-stranded when the temperature is lowered. Therefore, by controlling the denaturation and renaturation of DNA through temperature changes, adding designed primers, DNA polymerase, and dNTP can complete the in vitro replication of specific genes. [0003] PCR (Polymerase Chain Reaction) uses DNA denaturation in vitro at a high temperature of 95 °C to b...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12M1/38C12M1/00
Inventor 王淑军杨锦宇管雷
Owner 杨锦宇
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