Tear test method based on surface-enhanced raman spectroscopy

A technology of surface-enhanced Raman and spectroscopy, which is applied in the field of tear testing based on surface-enhanced Raman spectroscopy, and can solve problems such as difficulty in implementation

Inactive Publication Date: 2014-02-26
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for some substances with extremely low content or the target detection is the simultaneous detection of all tear components, the above methods are difficult to achieve

Method used

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  • Tear test method based on surface-enhanced raman spectroscopy
  • Tear test method based on surface-enhanced raman spectroscopy
  • Tear test method based on surface-enhanced raman spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Synthesis of nanoparticles: 55nm gold nanoparticles were synthesized by the Frens method.

[0032] (2) Acquisition of the measured tears: Under a slit lamp microscope, the field of view is magnified 16X, and a sterile capillary tube with a 0.3 mm aperture siliconized is placed in the tear river of the lower eyelid to avoid eye irritation. Capillaries draw tears into the capillaries by means of siphon force. Use an eppendorf gun to blow the collected tears into a 1.5ml centrifuge tube. The collected tear samples can be used immediately or stored in a -80°C refrigerator for use within two weeks.

[0033] (3) Mix the nanoparticles obtained in steps (1) and (2) with tear fluid components: use an eppendorf gun to quantitatively take 1.5ul tear fluid and gold nanoparticles, mix them, and mix well.

[0034] (4) Direct detection in the capillary: use the capillary to suck the mixture of tear fluid and silver nanoparticles in the above mixed step (3).

[0035] (5) Acquired...

Embodiment 2

[0038] (1) Synthesis of nanoparticles: 55nm silver nanoparticles were synthesized by the Frens method.

[0039] (2) Acquisition of the measured tears: Under a slit lamp microscope, the field of view is magnified 16X, and a sterile capillary tube with a 0.3 mm aperture siliconized is placed in the tear river of the lower eyelid to avoid eye irritation. Capillaries draw tears into the capillaries by means of siphon force. Use an eppendorf gun to blow the collected tears into a 1.5ml centrifuge tube. The collected tear samples can be used immediately or stored in a -80°C refrigerator for use within two weeks.

[0040] (3) Mix the nanoparticles obtained in steps (1) and (2) with tear fluid components: use an eppendorf gun to quantitatively take 1.5ul of tear fluid and silver nanoparticles, mix them, and mix well.

[0041] (4) Direct detection in the capillary: use a capillary to drop the tears and silver nanoparticles in the above mixed step (3) on the silicon wafer, and form rou...

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PUM

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Abstract

The invention discloses a tear test method based on surface-enhanced raman spectroscopy. The tear test method comprises the steps of mixing nano particles and tears, and detecting a tear and particle mixture through a raman spectrometer, thus obtaining a tear component raman spectrogram, wherein the tear and particle mixture can be liquid, semi-liquid or a dried crystal. The invention aims at providing a novel, quick, noninvasive and sensitive method for detection and analysis of the tears.

Description

technical field [0001] The invention belongs to the field of biological sample testing methods, in particular to a tear fluid testing method based on surface-enhanced Raman spectroscopy. Background technique [0002] The tear film is composed of lipids secreted by meibomian glands, watery fluid secreted by lacrimal glands, and mucin secreted by epithelial cells on the ocular surface. The tear fluid secreted by the lacrimal gland and its accessory glands can spread to the entire surface of the eyeball through blinking movements to lubricate the surface of the eye and supply the substances needed for the normal metabolism of the epithelial cells on the ocular surface to maintain the normal function of the ocular surface tissue. Normal tear film components are complex and low in content, including lysozyme, lactoferrin, albumin, epidermal growth factor, β-type transforming growth factor, wax ester, cholesterol ester, polar fat, sodium, potassium, etc. Currently, commonly used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 李炜任斌张丽颖胡佩邹笃雷刘祖国
Owner XIAMEN UNIV
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