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Method for accelerating rooting of masson pine tissue culture secondary sprout

A technology of subcultured buds and masson pine, applied in the field of promoting the rooting of masson pine tissue culture subcultured shoots, tissue culture seedlings, can solve the problem of low rooting rate of single buds, to improve the rooting situation of subcultured buds, good economic benefits and social benefits, the effect of promoting the rooting situation of subculture shoots

Inactive Publication Date: 2014-03-05
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many scholars have shown in the past that rooting with single buds of Pinus massoniana cultured in the first generation can usually obtain a relatively ideal rooting rate, but after subculture, the single-bud rooting rate is obviously low

Method used

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  • Method for accelerating rooting of masson pine tissue culture secondary sprout

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Experimental materials

[0026] The terminal buds of aseptic seedlings germinated from mature cone seeds of Pine masson in the same year were used as explants, and the cones were collected from Ningming, Guangxi Ningming, Tongmian provenance, which is a high-yielding and excellent Pine masson family line Ningming No.

[0027] 2. Experimental method

[0028] (1) Cluster bud induction

[0029] Inoculate the sterile explants into the modified MS+6-BA 3.0mg·L -1 +NAA 0.1mg·L -1 +Sucrose 30000mg·L -1 + Agar 3500mg·L -1 cultured on culture medium. After 26 days of culture, the average cluster bud induction rate reached 99.1%.

[0030] The basic composition of the improved MS medium is: KNO 3 1650mg·L -1 ; NH 4 NO 3 600mg·L -1 ;CaCl 2 2H 2 O260mg·L -1 ;MgSO 4 ·7H 2 O370mg·L -1 ; Ca(NO 3 ) 2 4H 2 O400mg·L -1 ;KH2 PO 4 170mg·L -1 ;MnSO 4 ·H 2 O22.3mg·L -1 ; ZnSO 4 ·7H 2 O8.6mg·L -1 ; CuSO 4 ·5H 2 O0.025mg·L -1 ;H 3 BO 3 6.2mg·L -1 Na 2 MoO ...

Embodiment 2

[0044] 1. Experimental materials

[0045] The terminal buds of aseptic seedlings germinated from mature cone seeds of Pine masson in the same year were used as explants, and the cones were collected from Ningming pine family Ningming No.

[0046] 2. Experimental method

[0047] (1) Cluster bud induction

[0048] Inoculate the sterile explants into the modified MS+6-BA 4.0mg·L -1 +NAA 0.1mg·L -1 +Sucrose 30000mg·L -1 + Agar 3500mg·L -1 cultured on culture medium. After 26 days of culture, the average cluster bud induction rate reached 98.4%.

[0049] The basic composition of the improved MS medium is the same as in Example 1.

[0050] (2) Cluster bud elongation

[0051] Transfer the induced clustered buds to the improved MS+NAA 0.3mg·L -1 +Sucrose 30000mg·L -1 + Agar 3500mg·L -1 on elongation medium to promote elongation of the culture. After 28 days of elongation culture, the cluster buds are 3-5cm high.

[0052] (3) Subculture bud culture

[0053] Cut out the si...

Embodiment 3

[0063] 1. Experimental materials

[0064] The top buds of aseptic seedlings germinated from the mature cone seeds of Pine masson in the same year were used as explants, and the cones were collected from Ningming 7, a high-yielding and excellent Pine masson family in the mother forest of Pine masson in Ningming, Guangxi.

[0065] 2. Experimental method

[0066] (1) Cluster bud induction

[0067] Inoculate the sterile explants into the modified MS+6-BA 4.0mg·L -1 +NAA 0.1mg·L -1 +Sucrose 30000mg·L -1 + Agar 3500mg·L -1 cultured on culture medium. After 26 days of culture, the average cluster bud induction rate reached 99.0%.

[0068] The basic composition of the improved MS medium is the same as in Example 1.

[0069] (2) Cluster bud elongation

[0070] Transfer the induced clustered buds to the improved MS+AC 4000mg·L -1 +Sucrose 30000mg·L -1 + Agar 3500mg·L -1 on elongation medium to promote elongation of the culture. After 28 days of elongation culture, the cluste...

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Abstract

The invention discloses a method for accelerating the rooting of a masson pine tissue culture secondary sprout. The method comprises the following steps of independently intercepting the strongly-growing semi-lignified masson pine tissue culture secondary sprout with the height of 2-3cm; adopting different culture mediums and a two-step rooting method: firstly inoculating the sprout into a half improved MS culture medium IV which contains naphthylacetic acid, indolebutyric acid and saccharose, inducing adventitious root primordia 21-28d, and then transferring the sprout to a half improved MS culture medium V which contains active carbon or ascorbic acid and the saccharose without auxins to accelerate the expression and elongation of an adventitious root. According to the method, a rooted regenerated plant is transplanted into a peat soil, vermiculite and perlite mixed matrix to be cultured, so that the rooting condition of the secondary sprout is greatly improved; the rooting ratio of the masson pine secondary sprout achieves more than 82.5%, the highest rooting ratio achieves 90.6%, the root system quality is high, the transplantation survival rate achieves more than 85.1%, and the highest survival rate achieves 93.0%; good economic benefit and social benefit are achieved.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to tissue culture seedling raising technology, in particular to a method for promoting the rooting of pine tissue culture subculture shoots. Background technique [0002] Masson pine (Pinusmassonianan) is native to my country. It is one of the important tree species in barren hills greening, papermaking raw material forests, resin mining forests and natural landscape forests in southern my country. It is widely distributed in my country. As a tree species with high comprehensive utilization value and great promotion potential, masson pine can not only be used to produce three-board, papermaking and chemical fiber industry manufacturing, but also can be used to produce industrial raw materials such as rosin and turpentine. In recent years, with the intensification of contradictions between people's growing living needs and the shortage of timber resources and the gradual depl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 姚瑞玲吴幼媚蔡玲
Owner GUANGXI FORESTRY RES INST
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