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Method for promoting pinus massoniana tissue-cultured subcultured bud to root

A technique for substituting buds and masson pine, which is applied in the field of tissue culture and seedling raising and promoting the rooting of masson pine tissue culture subsequent shoots, can solve the problems of low rooting rate of single shoots, etc., so as to improve the situation of subsequent shoots and promote the growth of subsequence shoots. Rooting situation of shoots, the effect of good economic and social benefits

Active Publication Date: 2014-04-30
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many scholars have shown in the past that rooting with single buds of Pinus massoniana cultured in the first generation can usually obtain a relatively ideal rooting rate, but after subculture, the single-bud rooting rate is obviously low

Method used

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  • Method for promoting pinus massoniana tissue-cultured subcultured bud to root
  • Method for promoting pinus massoniana tissue-cultured subcultured bud to root
  • Method for promoting pinus massoniana tissue-cultured subcultured bud to root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Experimental materials

[0028] The terminal buds of aseptic seedlings germinated from the mature cone seeds of Pine masson in the same year were used as explants, and the cones were collected from Ningming 3, a high-yielding and excellent Pine masson family in the mother forest of Pine masson in Ningming, Guangxi.

[0029] 2. Experimental method

[0030] (1) Cluster bud induction

[0031] Inoculate the sterile explants into the modified MS+6-BA3.0mg·L -1 +NAA0.1mg·L -1 +Sucrose 30000mg·L -1 + Agar 3500mg·L -1 cultured on culture medium. After 26 days of culture, the average cluster bud induction rate reached 99.1%.

[0032] The basic composition of the improved MS medium is: KNO 3 1580mg·L -1 ; NH 4 NO 3 800mg·L -1 ;CaCl 2 2H 2 O260mg·L -1 ;MgSO 4 ·7H 2 O370mg·L -1 ; Ca(NO 3 )2 4H 2 O200mg·L -1 ;KH 2 PO 4 170mg·L -1 ;MnSO 4 ·H 2 O22.3mg·L -1 ; ZnSO 4 ·7H 2 O8.6mg·L -1 ; CuSO 4 ·5H 2 O0.025mg·L -1 ;H 3 BO 3 6.2mg·L -1 Na 2 MoO 4 2...

Embodiment 2

[0046] 1. Experimental materials

[0047] The terminal buds of aseptic seedlings germinated from mature cone seeds of Pine masson in the same year were used as explants, and the cones were collected from Ningming pine family Ningming No.

[0048] 2. Experimental method

[0049] (1) Cluster bud induction

[0050] Inoculate the sterile explants into the modified MS+6-BA4.0mg·L -1 +NAA0.1mg·L -1 +Sucrose 30000mg·L -1 + Agar 3500mg·L -1 cultured on culture medium. After 26 days of culture, the average cluster bud induction rate reached 98.4%.

[0051] The basic composition of the improved MS medium is the same as in Example 1.

[0052] (2) Cluster bud elongation

[0053] Transfer the induced clustered buds to the improved MS+NAA 0.3mg·L -1 +Sucrose 30000mg·L -1 + Agar 3500mg·L -1 on elongation medium to promote elongation of the culture. After 28 days of elongation culture, the cluster buds are 3-5cm high.

[0054] (3) Subculture bud culture

[0055] Cut out the sing...

Embodiment 3

[0065] 1. Experimental materials

[0066] The top buds of aseptic seedlings germinated from the mature cone seeds of Pine masson in the same year were used as explants, and the cones were collected from Ningming 7, a high-yielding and excellent Pine masson family in the mother forest of Pine masson in Ningming, Guangxi.

[0067] 2. Experimental method

[0068] (1) Cluster bud induction

[0069] Inoculate the sterile explants into the modified MS+6-BA4.0mg·L -1 +NAA0.1mg·L -1 +Sucrose 30000mg·L -1 + Agar 3500mg·L -1 cultured on culture medium. After 26 days of culture, the average cluster bud induction rate reached 99.0%.

[0070] The basic composition of the improved MS medium is the same as in Example 1.

[0071] (2) Cluster bud elongation

[0072] Transfer the induced clustered buds to the improved MS+NAA, say 0.4mg·L -1 +Sucrose 30000mg·L -1 + Agar 3500mg·L -1 on elongation medium to promote elongation of the culture. After 28 days of elongation culture, the clu...

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Abstract

The invention discloses a method for promoting a pinus massoniana tissue-cultured subcultured bud to root. The method comprises the following steps: selecting the robust and half lignified pinus massoniana tissue-cultured subcultured bud with a seedling height of 2-3 cm; by adopting different culture media in a two-step rooting method, inoculating the subcultured bud in a 1 / 2 modified MS culture medium IV that contains indoleacetic acids, indolebutyric acids and sucrose; conducting induction treatment on an adventitious root primordium 21-28 d; transplanting the subcultured bud to a 1 / 2 modified MS culture medium V that contains activated carbon or ascorbic acids, and sucrose, without auxins, in order to promote the adventitious root to express and extend; transplanting a rooted regeneration plant to a mixed matrix that contains yellow soil or peat soil, vermiculite and perlite for cultivating. According to the invention, the method can tremendously promote and improve the rooting condition of the subcultured bud, the rooting rate of the pinus massoniana subcultured bud is above 93.1%, and the highest rooting rate is as high as 97.6%; the root system quality is higher; the survival rate of transplanting is above 85.1%, and the highest survival rate of transplanting is as high as 93.0%; the method has good economic benefits and social benefits.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to tissue culture seedling raising technology, in particular to a method for promoting the rooting of pine tissue culture subculture shoots. Background technique [0002] Masson pine (Pinus massonianan) is native to my country. It is one of the important tree species in the south of my country for barren hills greening, papermaking raw material forests, resin mining forests, and natural landscape forests. It is widely distributed in my country. As a tree species with high comprehensive utilization value and great promotion potential, masson pine can not only be used to produce three-board, papermaking and chemical fiber industry manufacturing, but also can be used to produce industrial raw materials such as rosin and turpentine. In recent years, with the intensification of contradictions between people's growing living needs and the shortage of timber resources and the gradu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 姚瑞玲吴幼媚蔡玲
Owner GUANGXI FORESTRY RES INST
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