Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of smelly goat extract and its preparation method and application

A kind of smelly goat and extract technology, applied in the field of smelly goat extract and preparation thereof

Active Publication Date: 2016-06-08
GUIZHOU INST OF TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Smelly goat (Orixajaponica Thunb.), also known as stinky seedlings, smelly Changshan, and big goat, is a plant of Rutaceae. Volatile substances, there are few reports on the medicinal value of stinky goats
The application in the prevention and treatment of tuberculosis has never been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of smelly goat extract and its preparation method and application
  • A kind of smelly goat extract and its preparation method and application
  • A kind of smelly goat extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation method of smelly goat extract:

[0024] (1) Take the roots, stems and leaves of the stinky goat plant as raw materials, air-dry them naturally, crush them through a 30-mesh sieve, and obtain the stinky goat fine powder;

[0025] (2) Take 426.24g of smelly goat fine powder, add 860ml of 90%-99%V / V ethanol, extract under the action of ultrasonic waves, the ultrasonic frequency is 40KHz, the extraction temperature is 40°C, the extraction time is 40min, and the same volume is replaced Ethanol, repeatedly leached three times, combined three extractions;

[0026] (3) Filter the extract, and recover ethanol from the filtrate under reduced pressure to obtain 16.65 g of ethanol extract;

[0027] (4) Take 4.24g of ethanol extract, add 5ml of hydrochloric acid or tartaric acid of pH 1-3 to dissolve in water, add 25ml of ethyl acetate to extract, extract 5 times, combine ethyl acetate extracts; concentrate under reduced pressure to recover ethyl aceta...

example 2

[0029] Example 2: The drug susceptibility test of stinky goat ethanol extract ethyl acetate acid extract and ethyl acetate alkali extract comprises the following steps:

[0030] (1) Improved Roche culture medium: according to the national standard GB15987-1995, the improved Roche culture was prepared as a blank control;

[0031] (2) Roche medium containing isoniazid: Add isoniazid to the modified Roche medium, and the final concentration of isoniazid in the medium is 0.2 μg / ml, which is used as a negative control;

[0032] (3) Extract-containing Roche medium: After preparing the improved Roche medium base according to the national standard GB15987-1995, add ethanol extract to prepare extract medium, ethyl acetate acid extract medium, ethyl acetate alkali extraction Dilute the amount of the medium extract, the concentration gradient of the ethanol extract is Y1-Y9, a total of 9 concentration gradients, make 10 parallels for each concentration, and store in a -4°C refrigerator a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The stinky goat extract and its preparation method and application of the present invention relate to Chinese herbal medicine extracts and applications thereof. The roots, stems and leaves of the stinky goat plant are used as raw materials, naturally air-dried, crushed, extracted with ethanol, and solvent ethanol is recovered to obtain Ethanol extract, the ethanol extract is dissolved in hydrochloric acid or tartaric acid water with pH 1-3, extracted with ethyl acetate, and ethyl acetate is recovered to obtain ethyl acetate acid extract, adjusted to pH 9-11 with water, and then extracted with ethyl acetate , reclaim ethyl acetate to obtain ethyl acetate base extract; smelly goat ethanol extract, ethyl acetate acid extract and ethyl acetate base extract are all brown pastes, scanned by UV-visible spectrophotometry at a wavelength of 630- There is a common absorption peak between 710nm, and all three have good activity of inhibiting Mycobacterium tuberculosis.

Description

technical field [0001] The present invention relates to Chinese herbal medicine extract and its application, in particular to smelly goat extract and its preparation method and application. Background technique [0002] Smelly goat (Orixajaponica Thunb.), also known as stinky seedlings, smelly Changshan, and big goat, is a Rutaceae plant that has the functions of dispelling wind and clearing heat, promoting qi and blood circulation, detoxifying and dehumidifying, cutting off malaria, and analgesic effects. The main chemical components are alkaloids and Volatile substances, there are few reports on the medicinal value of stinky goats. The application in tuberculosis control has never been reported. As a difficult chronic infectious disease in human history, tuberculosis has remained popular until recent years, and its pathogenic source is Mycobacterium tuberculosis. In order to further explore the medicinal value of the smelly goat, the inventor of the present invention use...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/75A61P31/06
Inventor 张振李岑骆科文赵伟欧维正闫岩王燕
Owner GUIZHOU INST OF TECH