Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of soluble recombinant protein for promoting the growth of large yellow croaker and its preparation

A recombinant protein and soluble technology, applied in the fields of molecular biology and aquaculture, can solve the problems of slow growth, affecting the supply and demand relationship in the large yellow croaker market, and the deterioration of meat quality and flavor, and achieve the effect of promoting seedling growth and good application prospects

Inactive Publication Date: 2016-02-24
NINGBO UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, large yellow croaker, as the main marine economic species in Zhejiang Province, has experienced a series of economic degradation phenomena, premature sexual maturity, slow growth, small broodstock, and poor meat flavor after years of artificial cage and earthen pond culture. The problem directly affects the market supply and demand of large yellow croaker

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of soluble recombinant protein for promoting the growth of large yellow croaker and its preparation
  • A kind of soluble recombinant protein for promoting the growth of large yellow croaker and its preparation
  • A kind of soluble recombinant protein for promoting the growth of large yellow croaker and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] Sample Collection

[0022] The large yellow croaker skeletal muscle sample was taken from the back of the first-year-old large yellow croaker above the lateral line and below the dorsal fin of Zhejiang Ningbo Xiangshan Harbor Seedling Co., Ltd., packed in a 1.5ml RNasefree centrifuge tube, quickly put into liquid nitrogen, and brought back to the laboratory for storage at -70 ℃ for the extraction of total RNA.

[0023] Reagents related to RNA manipulation

[0024] Trizol reagent: purchased from Sangon Bioengineering (Shanghai) Co., Ltd.;

[0025] Chloroform, absolute ethanol (AR grade) and isopropanol: all purchased from Ningbo Boao Scientific Instrument Co., Ltd.;

[0026] 70% ethanol: take 70ml of absolute ethanol, add RNaseFree-H 2 O was adjusted to 100ml, and stored at 4°C for later use;

[0027] RNaseFree-H 2 O: purchased from Sangon Bioengineering (Shanghai) Co., Ltd.;

[0028] Chloroform: isoamyl alcohol (24:1): Mix 48ml of chloroform and 2ml of isoamyl alc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a soluble recombinant protein for promoting the growth of large yellow croaker and a preparation method of the soluble recombinant protein. The soluble recombinant protein has an amino acid sequence shown in SEQ ID NO.2. The protein provided by the invention is capable of effectively promoting the growth of large yellow croaker fries and has good application prospect.

Description

technical field [0001] The invention belongs to the fields of molecular biology and aquaculture, and in particular relates to a soluble recombinant protein for promoting the growth of large yellow croaker and a preparation method thereof. Background technique [0002] Large yellow croaker (Larimichthyscrocea, Richadson), commonly known as large yellow croaker, red melon, sweet-scented osmanthus yellow croaker, rockfish, stonefish, etc., is an important economically farmed fish in my country and belongs to one of the four major marine products in China. From a taxonomic point of view, large yellow croaker belongs to Animalia, Chordata, Vertebrata, Osteichthyes, Actinopterygii, Perciformes (Perciformes), Totoaba (Sciaenidae), Yellow Fish (Pseudosciaena). It is mainly distributed in the coast of mainland China from the south of the central Yellow Sea to the east of the Qiongzhou Strait, and the subtropical region of the Northwest Pacific Ocean (Santose et al., 2013). Min-Yued...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/495C12N15/12C12N15/10C12N15/70A01K61/00
CPCY02A40/81
Inventor 薛良义应俊
Owner NINGBO UNIV