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Shepherdia argentea caulicle regenerated plant induction culture medium and method for performing plant culture by utilizing culture medium

A technique of induction medium and silver buffalo fruit, which is applied in the field of plant tissue culture, can solve the problems of low differentiation rate and low rooting rate of cultured plants, and achieve the effect of obvious proliferation, increased differentiation rate, and rapid propagation of excellent seedlings

Inactive Publication Date: 2014-03-19
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention is to solve the low rooting rate of the existing silver buffalo fruit asexual cutting method propagation, and the technical problem that the differentiation rate of the plant is cultivated with MS medium. 1 ) and Silver Buffalo Plant Culture Method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0013] Specific embodiment one: the silver buffalo young stem regeneration plant induction medium of the present embodiment (recorded as SN 1 ) from 400 to 450 mg of NH per liter 4 NO 3 , 900~920mg of KNO 3 , 170~190mg of KH 2 PO 4 , 370~395mg of MgSO 4 ·7H 2 O, 100~110mg of CaCl 2 2H 2 O, 6.2-6.5 mg of H 3 BO 3 , 22.3~24.3mg of MnSO 4 4H 2 O, 8.6~9.2mg of ZnSO 4 ·7H 2 O, 0.25~0.3mg of Na 2 MoO 4 2H 2 O, 0.025~0.03mg of CuSO 4 ·5H 2 O, 0.025-0.03mg of CoCl 2 ·6H 2 O, 37.3-37.9 mg of Na 2 -EDTA, 27.8-28.2 mg of FeSO 4 ·7H 2 O, 100~105mg of C 6 h 12 o 6 , 0.5~0.7mg of C 6 h 5 NO 2 , 0.1 ~ 0.15mg of C 12 h 17 ClN4 OS·HCl, 0.5-0.6 mg of C 8 h 11 o 3 N·HCl, 2~2.5mg of C 2 h 5 NO 2 , 0.5-2.0mg of 6-benzylaminopurine (6-BA), 0.05-0.5mg of zeatin (ZT) and 0.05-0.5mg of naphthaleneacetic acid (NAA), 20-25g of sucrose, 6-7g of agar and The remainder consisted of distilled water.

[0014] Ammonium nitrate (NH 4 NO 3 ), potassium nitrate (KNO 3 ), ...

specific Embodiment approach 2

[0016] Specific embodiment two: what this embodiment is different from specific embodiment one is that every liter of silver buffalo young stem regeneration plant induction medium is made up of 400mg of NH 4 NO 3 , 900mg of KNO 3 , 170mg of KH 2 PO 4 , 370mg of MgSO 4 ·7H 2 O, 100mg of CaCl 2 2H 2 O, 6.2mg of H 3 BO 3 , 22.3mg of MnSO 4 4H 2 O, 8.6 mg of ZnSO 4 ·7H 2 O, 0.25 mg of Na 2 MoO 4 2H 2 O, 0.025mg of CuSO 4 ·5H 2 O, 0.025mg of CoCl 2 ·6H 2 O, 37.3 mg of Na 2 - EDTA, 27.8 mg of FeSO 4 ·7H 2 O, 100mg of C 6 h 12 o 6 , 0.5mg of C 6 h 5 NO 2 , 0.1mg of C 12 h 17 ClN 4 OS·HCl, 0.5 mg of C 8 h 11 o 3 N·HCl, 2mg of C 2 h 5 NO 2 , 1.0 mg of 6-benzylaminopurine, 0.1 mg of zeatin, 0.05 mg of naphthalene acetic acid, 20 g of sucrose, 6 g of agar and the remainder of distilled water.

[0017] Using the silver buffalo young stem regenerated plant induction medium of this embodiment to cultivate the silver buffalo young stem regenerated plant, ...

specific Embodiment approach 3

[0018] Specific embodiment three: what this embodiment is different from specific embodiment one or two is that every liter of silver buffalo young stem regeneration plant induction medium consists of 400mg of NH 4 NO 3 , 900mg of KNO 3 , 170mg of KH 2 PO 4 , 370mg of MgSO 4 ·7H 2 O, 100mg of CaCl 2 2H 2 O, 6.2mg of H 3 BO 3 , 22.3mg of MnSO 4 4H 2 O, 8.6 mg of ZnSO 4 ·7H 2 O, 0.25 mg of Na 2 MoO 4 2H 2 O, 0.025mg of CuSO 4 ·5H 2 O, 0.025mg of CoCl 2 ·6H 2 O, 37.3 mg of Na 2 - EDTA, 27.8 mg of FeSO 4 ·7H 2 O, 100mg of C 6 h 12 o 6 , 0.5mg of C 6 h 5 NO 2 , 0.1mg of C 12 h 17 ClN 4 OS·HCl, 0.5 mg of C 8 h 11 o 3 N·HCl, 2mg of C 2 h 5 NO 2 , 2.0mg of 6-benzylaminopurine, 0.5mg of zeatin and 0.1mg of naphthaleneacetic acid, 20g of sucrose, 6g of agar and the remainder of distilled water.

[0019] Using the silver buffalo young stem regeneration plant induction medium of this embodiment to cultivate the silver buffalo young stem regeneration pl...

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Abstract

The invention discloses a shepherdia argentea caulicle regenerated plant induction culture medium and a method for performing plant culture by utilizing the culture medium, relates to a regenerated plant induction culture medium and a culture method, and aims to solve the technical problems of low rooting rate in a conventional shepherdia argentea clonal cutting culture method and low differentiation rate of plants cultured by an MS (murashige-skoog) culture medium. The culture medium consists of macroelement, trace element, iron salt, organic matters, hormones, sucrose and agar, wherein the hormones are 6-benzylaminopurine, zeatin and naphthalene acetic acid. The method comprises the following steps of collecting annual dormant shoots of shepherdia argentea from the second third of April and the first third of May, and performing hydroponic culture to obtain caulicles; washing and sterilizing the caulicles, inoculating the caulicles to the shepherdia argentea caulicle regenerated plant induction culture medium, placing the inoculated caulicles under a dark condition for culture after inoculation, and transferring the caulicles to a light condition for culture to obtain shepherdia argentea regenerated plants. According to the culture method, the tufted seedling inductivity reaches over 80 percent, meanwhile, the cost is lowered by 50 percent, and the seed nature purity is over 98 percent.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and is a special medium for plant induction of silver buffalo young stem regeneration. Background technique [0002] Silver buffalo fruit was introduced from Canada to the Daxinganling area of ​​Heilongjiang Province for planting in 2001. After being cultivated in the Daxinganling area, it can survive the winter safely and grow and bear fruit normally. [0003] Silver buffalo fruit (Shepherdia argentea) is a perennial shrub of the genus Shepherdia argentea, native to northern America, with a plant height of 5.5 meters, small, yellow flowers, flowering in mid-spring, dense leaf texture, and silvery, narrow leaves , 2-6cm long, deciduous in autumn, leaf color unchanged, fruit red to orange red. Silver buffalo fruit has excellent characteristics such as resistance to atmospheric drought, salt and alkali, soil poverty, and pest resistance. The lowest temperature in the distribution area...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王承义李晶宋国华孙一萌王丹徐永波梁鸣林玉秋
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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