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Pretreatment method of blood sample

A blood sample and pretreatment technology, applied in the medical field, can solve problems such as cumbersome processes, and achieve the effects of simplifying preprocessing steps, saving preprocessing time, and reducing dependencies

Active Publication Date: 2014-03-19
NAVY MEDICINE RES INST OF PLA
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Problems solved by technology

[0006] The problem solved by the present invention is that the process of the traditional blood sample pretreatment method is too cumbersome

Method used

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  • Pretreatment method of blood sample

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Embodiment Construction

[0029] The invention provides a blood sample pretreatment method, which has simple operation, few steps and saves time.

[0030] In order to make the above objects, features and advantages of the present invention more comprehensible, specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0031] The first step is to take a clean test tube and add acetonitrile solvent and plasma to the test tube. The volume ratio of plasma to acetonitrile is greater than or equal to 1:3. Under the action of acetonitrile solvent, the impurities in the plasma are separated to form flocs. The desmosin is attached in the ionic state in the floc, which is in the solution in the test tube. In this embodiment, the plasma volume ranges from 75 μL to 100 μL. Compared with the prior art, the volume of plasma required in this embodiment is very small.

[0032] In this example, the volume ratio of plasma to acetonitrile solvent is 1...

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Abstract

The invention discloses a pretreatment method of a blood sample. The method comprises the following steps: adding an acetonitrile solvent and plasma to a test tube, wherein the volume ratio of the plasma to the acetonitrile solvent is greater than or equal to 1:3; separating foreign proteins in the plasma to form a floccule; attaching desmosine in the floccule; extracting the floccule; adding a high-performance liquid chromatography acidic water solution to the floccule, wherein the desmosine in the floccule drifts away in the high-performance liquid chromatography acidic water solution in an ionic form; extracting the high-performance liquid chromatography acidic water solution with desmosine ions; attaching the desmosine in the floccule; adding subsequent floccule to the high-performance liquid chromatography acidic water solution; dissolving the desmosine and extracting the high-performance liquid chromatography acidic water solution with the desmosine ions for high-performance liquid chromatography (HPLC) detection. The pretreatment method of the blood sample in the technical scheme does not need the steps of extraction, nitrogen drying and the like in the prior art, the pretreatment step of the blood sample is simplified, the pretreatment time is saved, an operator is not needed to monitor in real time, and the dependency on people is reduced.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a blood sample pretreatment method. Background technique [0002] Preprocessing the blood sample refers to extracting the analyte from the blood sample. In the follow-up, the purpose of disease inspection and health inspection will be achieved by detecting the content, composition and other parameters of the analyte. [0003] For example, in the prior art, the specific product desmosine (desmosine, DES) is generated in patients with lung injury due to the degradation of the rich elastic fiber tissue in the lung, resulting in an increase in the concentration of desmosine in the plasma. Therefore, use high performance liquid chromatography (High Performance Liquid Chromatography, HPLC) to detect the concentration of DES in plasma, and according to the concentration of DES, the clinical examination and research of lung injury can be realized. However, HPLC has strict requirements...

Claims

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Application Information

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IPC IPC(8): G01N30/06
Inventor 许骥陈杰陈锐勇刘晓波何佳陈新向导
Owner NAVY MEDICINE RES INST OF PLA
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