Method for extracting small molecule peptide from fresh animal bone

Abstract

A method for extracting a small molecule peptide from a fresh animal bone is provided. The technological process of the method comprises the following steps: cleaning and breaking a fresh cattle or sheep bone, autoclaving, adding a special bone enzyme to conduct enzymolysis, deodorizing, filtering, concentrating, spraying the power to dry, and packaging a finished product. The method is characterized in that the temperature is higher than 120 DEG C, the pressure is 0.15-0.16 MPa and the fresh cattle or sheep bone is cooked for 8 h in the step of autoclaving; in the step of enzymolysis, the temperature is reduced to 54-56 DEG C after raising the temperature and sterilizing, NaOH is added to regulate the pH to 7.0, then the 0.5% special bone enzyme comprising an incision enzyme, an excision enzyme, a flavor enzyme and a pancreatic enzyme is added to conduct enzymolysis for 6 h; in the step of deodorizing, activated carbon is used; in the step of filtering, a candle filter and then a barrel filter are used; in the step of concentrating, the concentration pressure is -0.4 MPa to -0.6 MPa, and the concentration temperature is between 75 DEG C to 80 DEG C. The method has the advantages that the protein in the animal bone is hydrolyzed through bio-enzymes into all water-soluble nutritional ingredients, a product is high in the content of the small molecule peptide and the solubility, is favorable for absorbing by a human body, and is suitable for extracting the small molecule peptide of various animal bones.

Description

technical field [0001] The invention belongs to a protein extraction method, in particular to a method for extracting small-molecule peptides from the bone by enzymatically using protein-containing bovine and sheep animal bones as a base material. Background technique [0002] In recent years, science believes that the human body absorbs protein mainly in the form of peptides. Peptides are molecules composed of amino acids with a molecular weight between 5000-200 Daltons, and those with molecular weights below 1000 Daltons are called small molecular peptides. , generally composed of 2-6 amino acids. The difference between small molecule peptides and other peptides is that the molecular weight is smaller, and it does not need to be digested in the human body, so it is convenient for direct absorption. [0003] At present, the method of using biological enzymes to catalyze proteins to synthesize bioactive peptides has been widely used. There are several methods for extracting...

AI Technical Summary

Problems solved by technology

[0003] At present, the method of using biological enzymes to catalyze proteins to synthesize bioactive peptides has been widely used. There are several methods for extracting peptides from bone protein. The basic method is to cook fresh animal bones for a long time under high temperature and high pressure. Minerals, crude protein, and oil are completely separated from the bone, and then the protein is enzymatically hydrolyzed, and edible alcohol is used to activate trypsin for hydrolysis. The disadvantage of this method is that the molecular weight of the polypeptide after enzymolysis is also large, and the solubility is not good. At the same time, the color is dark, the smell is heavy and it is difficult to remove, so it is not easy to be widely used as a nutritional product

At present, there is a lack of animal bone peptides with higher content of small molecule peptides, better color, taste and solubility

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