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Kits for preparing decellularized liver scaffolds and methods of use thereof

A kit and decellularization technology, applied in medical science, prosthesis, etc., can solve the problems of unsatisfactory preparation effect, imperfect preparation method of decellularized liver scaffold, etc., and achieve stable effect of decellularization of liver cells.

Active Publication Date: 2016-06-29
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current preparation methods for decellularized liver scaffolds are not perfect, and the preparation effect is not ideal.

Method used

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  • Kits for preparing decellularized liver scaffolds and methods of use thereof
  • Kits for preparing decellularized liver scaffolds and methods of use thereof
  • Kits for preparing decellularized liver scaffolds and methods of use thereof

Examples

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Embodiment 1

[0041] Porcine liver is similar in size to human liver and is easy to obtain. It is the most suitable scaffold material for constructing tissue engineering liver. Taking pig liver as an example, the kit for preparing decellularized liver scaffold and its use method are described in detail below.

[0042] The kit of this embodiment includes: thrombolytic solution, perfusion solution I, perfusion solution II, perfusion solution III, and perfusion solution IV.

[0043] Among them, the components and concentrations of the thrombolytic solution are: urokinase 100000IU / L, NaCL9g / L.

[0044] The components and concentrations of perfusion fluid I are: EDTA 0.2g / L, NaCL 9g / L.

[0045] The components and concentrations of the perfusate II are: sodium lauryl sulfate 5g / L and NaCL 9g / L.

[0046] The composition and concentration of perfusion solution III: DNaseI 0.01g / L.

[0047] The composition and volume concentration of the perfusate IV is: peracetic acid 0.01%.

[0048] In this embodiment, the s...

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Abstract

The invention discloses a kit for preparing an acellularized liver scaffold. The kit comprises a thrombolytic liquid, a perfusate I, a perfusate II, a perfusate III and a perfusate IV. The thrombolytic liquid comprises the following components: urokinase 100000IU / L, NaCL 9g / L; the perfusate I comprises the following components by concentration: EDTA (Ethylene Diamine Tetraacetic Acid) 0.2g / L and NaCL 9g / L; the perfusate II comprises the following components: lauryl sodium sulfate 5g / L and NaCL 9g / L; the perfusate III comprises the following component by concentration: Dnase I 0.01g / L; the the perfusate IV comprises the following components: peracetic acid 0.01%. The thrombolytic liquid, the perfusate I, the perfusate II, the perfusate III and the perfusate IV are orderly applied to perfusing of the liver so that the acellularized liver scaffold can be prepared; the method is stable in liver decellularization effect and capable of keeping complete ingredients such as the extracellular matrix structure and the collagen.

Description

Technical field [0001] The invention relates to the field of liver tissue engineering, in particular to a kit for preparing decellularized liver scaffolds and a method of use thereof. Background technique [0002] Under the background that the existing liver replacement therapy is still insufficient, it is necessary to develop a tissue engineered liver for transplantation. Liver tissue engineering is an innovative way to construct a transplantable liver. Its core content is to construct a transplantable tissue engineered liver by compounding liver seed cells with bio-scaffold materials, which can be used to perform morphological, structural and Permanent replacement of functions. At present, due to the lack of suitable scaffold materials, the tissue engineered liver is usually constructed with simple structure and lacks a complete blood vessel network to achieve the purpose of liver replacement. [0003] The performance and structural requirements of an ideal liver scaffold mater...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
Inventor 程远潘明新汪艳李阳高毅
Owner ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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