Technique for cleaving out part of poly(a) chain and/or 3'-terminal sequence of mRNA to inhibit translation reaction

Abstract

Provided is an artificial gene expression inhibition method which can be designed more readily compared with conventional methods (an RNAI method, a ribozyme method, an antisense method), and in which the effect thereof can be confirmed readily. Provided are: a method for inhibiting the translation reaction of a target gene, which comprises cleaving out a part of a poly(A) chain and / or a 3'-terminal sequence of target mRNA; a kit for inhibiting a translation reaction, which comprises a reagent capable of cleaving out a part of a poly(A) chain and / or a 3'-terminal sequence of target mRNA; a cell into which a reagent capable of cleaving out a part of a poly(A) chain and / or a 3'-terminal sequence of target mRNA is introduced so as to inhibit the translation reaction of a target gene; and a non-human living organism into which a reagent capable of cleaving out a part of a poly(A) chain and / or a 3'-terminal sequence of target mRNA is introduced so as to inhibit the translation reaction of a target gene.

Description

【Technical field】 [0001] The present invention relates to the technology of cutting off the poly(A) chain and / or part of the 3' end sequence of the target mRNA, thereby inhibiting the translation reaction of the target gene. 【Background technique】 [0002] As methods for artificially restricting gene expression, there are RNAi method (Patent Document 1, Non-Patent Document 1), ribozyme method (Patent Document 2, 3, Non-Patent Document 2), and antisense method (Patent Document 4, 5, Non-Patent Document 2). Patent Document 3). The RNAi method or ribozyme method is a method of inhibiting gene expression by inducing specific degradation of target mRNA by nucleic acid or the like that specifically binds to target mRNA. The antisense method is a method of inhibiting gene expression by inducing the inhibition of translation reaction or inhibiting the normal splicing reaction by duplexing the target mRNA by a nucleic acid or the like that specifically binds to the target mRNA. [...

AI Technical Summary

Problems solved by technology

[0004] On the other hand, in the antisense method, although the target sequence is limited to the periphery of the translation initiation codon and the splice junction, the former cannot detect the antisense effect at the mRNA level, so the judgment of the confirmation effect is cumbersome

In addition, although the effect of the latter can be detected at the mRNA level, it is difficult to evaluate whether it is a protein that has lost function

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