Application of water-soluble lachnum melanin in preparing medicine for inhibiting liver cancer

A technology of Trichodia granulosus and inhibition of liver cancer, applied in Trichodia granulosa melanin in the field of medicine

Inactive Publication Date: 2014-04-16
HEFEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of water-soluble lachnum melanin in preparing medicine for inhibiting liver cancer
  • Application of water-soluble lachnum melanin in preparing medicine for inhibiting liver cancer
  • Application of water-soluble lachnum melanin in preparing medicine for inhibiting liver cancer

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Embodiment 1

[0011] 1. Preparation of intracellular melanin (LIM) of Trichomonas granulosa:

[0012] The strain preservation number is CCTCC No: M2011054, which is inoculated into a 5L fermenter. The medium formula is 20% by mass percentage of potato juice, 2% of glucose, 1% of peptone, 0.03% of tyrosine, K 2 HPO 4 0.05%, MgSO 4 ·7H 2 O0.05% composition; the volume ratio of the loading factor is 0.7, the inoculum size is 8% (volume ratio), the culture temperature is 27°C, the ventilation rate is 1.0L / min, the stirring speed is 180r / min, and the fermentation time is 8 days ; Filtrate the fermentation broth, collect the mycelium, and dry it at 60°C. The ratio of the mass of mycelium (g) to sodium hydroxide (mL) with a concentration of 0.4mol / L is 1:200 (g / mL) Mix well, extract in water bath at 80°C for 2 hours, and filter to obtain the extract; use 1mol / L HCl to adjust the pH of the above extract to 2, and let it stand for 12 hours to obtain a melanin suspension; then centrifuge the above...

Embodiment 2

[0056] First, the intracellular melanin of Trichodia granulosa was prepared and obtained according to the following conditions and methods: inoculate Trichodia granulosa singhingii with the strain preservation number CCTCC No: M2011054 in a 5L fermenter, and the medium formula is (by mass percentage): potato Juice 20%, Glucose 3%, Peptone 1.5%, Tyrosine 0.05%, K 2 HPO 4 0.06%, MgSO 4 ·7H 2 O0.04%; the volume ratio of the loading factor is 0.8, the inoculum size is 9% (volume ratio), the culture temperature is 26°C, the ventilation rate is 1.5L / min, the stirring speed is 160r / min, and the fermentation time is 10 days; Suction filter the fermentation broth, collect mycelia, dry at 60°C, and mix the mass of mycelia (g) with sodium hydroxide (mL) at a concentration of 0.5 mol / L at a ratio of 1:180 (g / mL). Evenly, leached in a water bath at 80°C for 3 hours, and suction filtered to obtain the extract; adjust the pH of the above extract to 2 with 1mol / L HCl, and let it stand for ...

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Abstract

The invention discloses an application of water-soluble lachnum melanin in preparing a medicine for inhibiting liver cancer. The application is characterized in that intracellular melanin generated by lachnum of which the strain preservation number is CCTCC (China Center for Type Culture Collection) No:M2011054 is modified by a histidine molecule to obtain water-soluble lachnum melanin. Pharmacodynamic experiments prove that the water-soluble lachnum melanin has a remarkable function of inhibiting liver cancer, and does not have toxic or side effect; the preparation method is simple and economical, so that the lachnum intracellular melanin of which the strain preservation number is CCTCC (China Center for Type Culture Collection) No:M2011054 and the water-soluble melanin thereof are subjected to a pharmaceutical conventional preparation method, and an effective dose of the water-soluble lachnum melanin and a proper amount of medical auxiliaries are mixed to prepare oral tablet or capsule type medicines for inhibiting liver cancer.

Description

technical field [0001] The invention belongs to the technical field of application of Trichodia granulosa melanin in the field of medicine, and specifically relates to the application of water-soluble melanin of Trichodia granulosa melanin with strain preservation number CCTCC No: M2011054 as a drug for inhibiting liver cancer. Background technique [0002] Malignant tumor is one of the common clinical diseases, and it is also one of the main causes of human death. At present, the treatment of malignant tumors mainly adopts methods such as radiotherapy and chemotherapy. The commonly used chemotherapeutic drugs are mostly chemically synthesized substances rather than natural products. Therefore, while killing tumor cells, they also have a certain degree of killing effect on normal cells; and simple Chemotherapy can cause loss of appetite and weight loss in patients, leading to weakening of the body, and causing a certain degree of immunosuppression, with relatively large toxi...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61K9/20A61K9/48A61P35/00
Inventor 叶明卢莹宋升
Owner HEFEI UNIV OF TECH
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