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Sponge fluid culture medium for rapid propagation of Myrciaria cauliflora tissue culture seedling and application of sponge fluid culture medium

A technology of liquid culture medium and sponge liquid, applied in the field of sponge liquid culture medium, can solve the problems of high cost of culture medium, low plant quality, low proliferation rate, etc., achieve easy cleaning, reduce production cost, and facilitate assembly and assembly Effect

Active Publication Date: 2014-04-23
LIUZHOU TIANZI HORTICULTURE
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0002] At present, the tissue culture of vine grapes mostly adopts the operation mode of simple liquid medium + solid medium, but the efficiency of this culture method is low, the proliferation rate is low, and the quality of vine tissue culture seedlings is not high. higher cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Preparation of sponge liquid medium:

[0017] Choose a cylindrical high-temperature-resistant filter sponge, the diameter of which is the same as the size of the bottom of the threaded bottle with a breathable cap, and the thickness of the sponge is 1cm. There are 15 V-shaped holes with a diameter of 0.8cm and a depth of 0.5cm on the sponge. The sponge block is placed flat on the bottom of the culture bottle with the perforated side facing up. Because no agar powder is added, the medicine can be prepared directly with tap water and the PH value can be adjusted by constant volume to form a liquid medium. Finally, an appropriate amount is divided into culture bottles with sponges, so that the nutrient solution has just soaked the sponge block. ;

[0018] (2) Sterilization of medium:

[0019] Since the selected sponge is a high-temperature-resistant material, the main component is polyurethane, which has the advantages of corrosion resistance, no toxic side effects, ...

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Abstract

The invention discloses a sponge fluid culture medium for rapid propagation of a Myrciaria cauliflora tissue culture seedling. Fifteen V-shaped pores with caliber of 0.8cm and depth of 0.5cm are formed on a sponge uniformly, the sponge is placed at the bottom of a culture bottle flatly, one pored side of the sponge faces up, chemicals are prepared by running water directly, the volume is metered and PH value is adjusted to form the fluid culture medium, the fluid culture medium is appropriately sub-packaged into the culture bottle with the sponge and justly soaks the sponge, and the fluid culture medium is 1 / 2MS culture medium. The sponge fluid culture medium has the beneficial effects that firstly, the Myrciaria cauliflora tissue culture material can fully absorb nutrition ingredients, the quality and the yield are improved and the reproduction rate can be increased by 5-6 times; secondly, the sponge can be used repeatedly, the fluid culture medium is sub-packaged and prepared to achieve convenience, and the bottle and the other tools can be cleaned easily; and thirdly, the expense for purchasing agar powder is saved and the production cost is reduced.

Description

technical field [0001] The invention relates to the field of rapid propagation of plant tissue culture seedlings, in particular to a sponge liquid medium for rapid propagation of grapevine tissue culture seedlings and an application thereof. Background technique [0002] At present, the tissue culture of vine grapes mostly adopts the operation mode of simple liquid medium + solid medium, but the efficiency of this culture method is low, the proliferation rate is low, and the quality of vine tissue culture seedlings is not high. higher cost. Contents of the invention [0003] Purpose of the present invention is exactly at the defect in the above-mentioned prior art, provides a kind of sponge liquid culture medium that can improve the proliferation rate of vine tissue culture. [0004] In order to achieve the above object, the technical scheme provided by the present invention is: the sponge liquid culture medium for the rapid propagation of the vine tissue culture seedling...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈财宝
Owner LIUZHOU TIANZI HORTICULTURE
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