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DNA (deoxyribonucleic acid)-barcode-based eel species identification method

A technology of species identification and barcode primers, which is applied in the field of eel species identification, can solve the problems of DNA barcode identification of eel species, etc., and achieve the effect of simple operation, strong specificity and short time consumption

Active Publication Date: 2014-04-23
INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Problems solved by technology

However, there is no report on the use of DNA barcodes to identify eel species at home and abroad.

Method used

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  • DNA (deoxyribonucleic acid)-barcode-based eel species identification method
  • DNA (deoxyribonucleic acid)-barcode-based eel species identification method
  • DNA (deoxyribonucleic acid)-barcode-based eel species identification method

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[0017] Reagent source: 10 × Taq buffer with Taq Enzymes and dNTPs were purchased from Shanghai Sangon Co., Ltd., primers were synthesized by Shanghai Sangon Co., Ltd., and DNA sequences were tested by Guangzhou Invetec Biotechnology Co., Ltd.

[0018] Primer synthesis

[0019] Japanese eel according to GenBank query Anguilla Japonica (AJ244813.1), American eel Anguilla rostrata (AJ244829.1), European eel Anguilla anguilla (AJ244826.1) Three kinds of eels

[0020] 16S rRNA coding gene sequence (16S rDNA), 3 pairs of PCR primers were automatically designed by Primer Premier 5.0, the amplified PCR products were sequenced, and 1 of the 3 eels with specific base sequences were selected from them. For the primers, the sequences are shown in Table 1.

[0021] Table 1 PCR primers and their annealing temperature

[0022]

[0023] The sequencing results of the PCR amplification products of the three eel samples were compared and verified in Genbank (Gene Bank), and they ...

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Abstract

The invention relates to a DNA (deoxyribonucleic acid)-barcode-based eel species identification method, in particular to a DNA-barcode-based species identification method for anguilla japonica, anguilla rostrata and auguilla anguilla. The method comprises the following steps of performing eel DNA extraction, performing PCR (polymerase chain reaction) amplification to obtain a 244bp product by utilizing DNA barcode primers, and performing species identification according to nucleotide sequence characteristic variation sites of the species, wherein the DNA barcode primers are EEL244-F:CCTGTTCC TCGTGGGGGC TTTT and EEL244-R:ATGGCATAACGAGGGTTTAACTG. The method is low in time consumption, simple and convenient to operate and high in specificity, and can be used for identifying common processed eel products such as roasted eel which cannot be morphologically identified, and technical support is provided for the maintenance of enterprises and consumer benefits and normal market order.

Description

technical field [0001] The present invention relates to a kind of eel species identification method based on DNA bar code, more specifically relate to a kind of Japanese eel (Japanese eel) based on DNA bar code Anguilla Japonica ), American eel ( Anguilla rostrata ) and European eel ( Anguilla anguilla ) species identification method. Background technique [0002] Fujian Province is a large province of eel farming, processing and exporting. The main products are three kinds of eels, namely Japanese eel ( Anguilla Japonica ), American eel ( Anguilla rostrata ), European eel ( Anguilla anguilla ), with an annual export volume of several billion yuan. Since eels are processed, such as grilled or cooked in other shapes, the species cannot be distinguished from the shape, and the prices of different types of eels are different. In order to prevent shoddy products and protect the interests of enterprises and consumers, a biological identification method for eel species ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q2563/185
Inventor 陈文炳邵碧英缪婷玉彭娟
Owner INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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