Modular columns and their uses
A chromatographic column and application technology, applied in the field of chromatography and chromatograph, can solve the problems of long elution time, uneconomical, large solvent consumption, etc., and achieve the effects of less solvent consumption, saving consumption, and easy and fast elution
Active Publication Date: 2016-06-22
NANJING NUOYUN BIOLOGICAL TECH CO LTD
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Problems solved by technology
This method takes a long time to elute, consumes a large amount of solvent, and requires relatively expensive fraction collection equipment when collecting
Especially for mixtures of relatively complex chemical monomer components, such as natural plant extracts, or chemical mixtures with less refined components, the above method is very uneconomical
Method used
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[0017] The present invention proposes a modularized chromatographic column aiming at the defect that the existing chromatographic column is a complete column, which is not fast enough and not economical enough in some application occasions. With this modular chromatographic column, once the mixture components are separated in the chromatographic column, the chromatographic column can be disassembled, and the separated components can be quickly obtained through lateral elution and collection, thereby shortening the elution time and reducing the amount of washing liquid .
[0018] Such as figure 1 As shown, the modular chromatographic column of the present invention is assembled by a top cover 1, a plurality of separation modules 2, a bottom cover 3 and a plurality of pressure rings 4 in sequence. The middle parts of the top cover 1 and the bottom cover 3 are respectively provided with through holes 11 and 31 for sample injection or sample discharge; at the same time, the washi...
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The invention discloses a modularized chromatographic column and purposes of the modularized chromatographic column; the modularized chromatographic column disclosed by the invention is formed by assembling a top sealing cover, a plurality of separation modules, a bottom sealing cover and a plurality of pressure rings having the sealing effect in sequence; the separation module is a circular pipe; a solid phase is filled in the separation module; sintered glass plates or films or nets are put at the upper and lower sides of the solid phase; various separation modules are connected detachably and fixedly in a manner of threading or clamping; and the topmost separation module, the bottommost separation module, the top sealing cover and the bottom sealing cover are connected detachably and fixedly in a manner of threading or clamping. By using the modularized chromatographic column disclosed by the invention, after sampling, elution is carried out by using lotion; once a mobile phase arrives at the discharging end or a sample is separated is observed or detected from outside in the elution process, elution can be stopped; then, the chromatographic column is detached from various joint parts; the separation modules are individually washed by using a solvent; and individual components can be obtained after being dried. The technical scheme provided by the invention is simple and easy in operation; the use amount of the solvent or lotion can be greatly saved; elution is rapider; furthermore, expensive fraction and collection equipment is not needed.
Description
technical field [0001] The present invention relates to methods and methods for separating or purifying certain chemical components of naturally occurring animals, plants, microorganisms and their metabolites, as well as various organic mixtures formed by humans in various production processes and scientific research processes. An instrument, in particular relates to chromatography and a chromatograph, and belongs to the technical field of separation and purification of organic mixtures. Background technique [0002] Chromatography, also known as "chromatography" or "chromatography", is an efficient separation technique for separating multi-component organic mixtures. In 1906, Russian botanist Mikhail Tsvet pioneered this separation technique. He poured the petroleum ether impregnation solution of plant chloroplast pigments into a thin straight glass column filled with calcium carbonate adsorbent, and then added pure petroleum Ether, let it flow down freely, the original mi...
Claims
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IPC IPC(8): B01D15/22
Inventor 朱惠霖
Owner NANJING NUOYUN BIOLOGICAL TECH CO LTD