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Marker for detecting colorectal cancer or esophageal cancer and method for inspecting same

An inspection method and marker technology, applied in biochemical equipment and methods, chemical instruments and methods, microbial measurement/inspection, etc., can solve problems such as time-consuming, high false positive rate, and difficulty in interpreting results

Inactive Publication Date: 2014-04-30
TORAY IND INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have disadvantages such as time-consuming determination of cancer, difficulty in interpreting results, and high false positive rate

Method used

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  • Marker for detecting colorectal cancer or esophageal cancer and method for inspecting same
  • Marker for detecting colorectal cancer or esophageal cancer and method for inspecting same
  • Marker for detecting colorectal cancer or esophageal cancer and method for inspecting same

Examples

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Effect test

Embodiment

[0115] The present invention will be described more specifically below by way of examples. However, the present invention is not limited by this example.

[0116]

[0117] (1) Production of hollow fiber filter

[0118] Bundle 100 polysulfone hollow fibers with a molecular weight cut-off of about 50,000 on the membrane surface, and fix the two ends on the glass tube with an epoxy resin-based encapsulant on the premise that the hollow part of the hollow fiber is not blocked. into micromodules. This miniature module (module A) can be used to remove high molecular weight proteins in serum or plasma, with a diameter of about 7 mm and a length of about 17 cm. A micromodule (module B) for concentrating low-molecular-weight proteins was similarly fabricated using a membrane with a pore size of molecular weight cut-off about 3 k. The micromodule has an inlet connected to the hollow fiber lumen at one end and an outlet at the other end. The inlet and outlet of the hollow fiber mic...

Embodiment 2

[0128] (1) Detection of COTL1 protein in esophageal cancer blood by Western blot

[0129]Plasma samples were obtained from 3 esophageal cancer patients (No. 1-3) and 4 healthy controls (No. 1-4) who obtained informed consent. 100 μL of Affi-Gel blue glue (Bio-Rad) and 50 μL of Protein A-Sepharose (GE Healthcare) were added to 100 μL of each sample, and reacted overnight at 4°C to remove albumin and immunoglobulin in the sample. The sample thus obtained was solubilized and boiled with SDS loading buffer (50 mM Tris-HCl, pH 6.8, 1 mM DTT, 5% SDS, 10% glycerol), and subjected to SDS-polyacrylamide gel (16%) electrophoresis, and then transfer the proteins to PVDF membranes. This was reacted with a rabbit anti-COTL1 polyclonal antibody (Proteintech Group) and a peroxidase-labeled secondary antibody (anti-rabbit IgG antibody). Immunoreactive proteins were visualized by sensitizing the X-ray film with SuperSignal West Femto Maximum Sensitivity Substrate (Pierce), and the signal int...

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PUM

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Abstract

This invention relates to a method for detecting or inspecting colorectal cancer or esophageal cancer with high detection sensitivity and accuracy and with minimal invasiveness to a subject. Specifically, the invention relates to a method for inspecting cancer, the method involving: measuring, in vitro, the COTL1 protein in a body fluid sample taken from a subject; and, on the basis of the amount of said protein, evaluating whether or not the subject is affected by cancer. The invention also relates to a kit for diagnosing colorectal cancer or esophageal cancer, the kit comprising an antibody that can specifically bind to said protein, or a fragment of the antibody.

Description

technical field [0001] The present invention relates to a method for examining colorectal cancer or esophageal cancer by measuring the concentration of COTL1 protein in body fluids as a marker for detection of colorectal cancer or esophageal cancer. [0002] The present invention also relates to a diagnostic kit for colorectal cancer or esophageal cancer comprising a substance capable of binding to the above protein for detecting colorectal cancer or esophageal cancer. Background technique [0003] Colorectal cancer is the cancer with the second most organ divisions, and 9.8% of all cancer patients are colorectal cancer patients. In 2008, 17.3 people per 100,000 people in the world suffered from colorectal cancer, and 8.2 people died of it. In Japan, colorectal cancer is the second most common cancer in all organs, and the number of patients tends to increase with the Westernization of food and lifestyle. [0004] Esophageal cancer was 7.0 per 100,000 people in the world i...

Claims

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Application Information

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IPC IPC(8): G01N33/68C07K16/18C12Q1/02G01N33/574
CPCG01N33/57419C07K16/3046C07K16/18C07K16/30G01N33/57496
Inventor 小林道元田中祥德高山爱子郑基晚坂井义治冈部宽
Owner TORAY IND INC
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