Induction method of mature embryo callus of syringa microphylla

A technology of lilac lobularis and callus, which is applied in the field of callus culture, can solve the problems of low induction rate and high callus contamination rate, and achieve the effects of high induction rate, good induction effect and long storage period

Inactive Publication Date: 2014-05-07
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of high pollution rate and low induction rate of callus induced by stems, leaves and root tips, and to provide a method for inducing mature embryo callus of Lilac lobularis

Method used

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  • Induction method of mature embryo callus of syringa microphylla
  • Induction method of mature embryo callus of syringa microphylla

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specific Embodiment approach 1

[0016] Specific embodiment one: the induction method of mature embryo callus of Lilac lobularis of the present embodiment, carry out according to the following steps:

[0017] 1. Disinfection and inoculation of explants: Take the mature seeds of Clove lobularis, remove the seed wings and rinse under running water for 10-14 hours, then disinfect them with 70%-75% alcohol for 25s-35s, and rinse them with sterile water Rinse 3 to 5 times, then sterilize with 1% to 3% NaClO for 25 to 35 minutes, rinse with sterile water for 3 to 5 times, cut from the middle of the seed under ultra-clean conditions, remove the radicle, Take out the zygotic embryo and separate the two cotyledons for later use;

[0018] 2. Induction of callus: The zygotic embryos treated in step 1 are induced by MS medium, and cultured in dark at a temperature of 23°C to 25°C and a humidity of 60% to 70%. , wherein the MS medium contains 1 mg / L of 6-BA, 1 mg / L of NAA, 3% by mass of sucrose and 0.7% by mass of agar, ...

specific Embodiment approach 2

[0023] Specific embodiment two: this embodiment is different from specific embodiment one in that: the mature seeds of cloves described in step one are healthy and mature seeds harvested in October of that year. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0024] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: after removing the seed wings described in step one, rinse under running water for 12 hours, and then disinfect with 70% alcohol for 30 seconds, Rinse 5 times with sterile water, then disinfect with 2% NaClO for 30 min, and rinse 5 times with sterile water. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses an induction method of the mature embryo callus of syringa microphylla, relates to a callus culture method, and particularly relates to an induction method of the mature embryo callus of syringa microphylla. The invention aims at solving the problems of high pollution rate and low induction rate of the callus induction using stems, leaves and root tips. The method disclosed by the invention comprises the following steps: (I) disinfection and inoculation of explants; (II) induction of callus. According to the method disclosed by the invention, the explant collection period is centralized, the pollution rate is controlled very low within 1%, and the induction rate of the syringa microphylla callus is high over 75%. The method disclosed by the invention is applied to callus induction.

Description

technical field [0001] The invention relates to a method for culturing callus, in particular to a method for inducing mature embryo callus of clove lobularis. Background technique [0002] Small-leaf lilac is a plant of the genus Syringaceae in the family Oleaceae. It is an excellent flowering shrub in northern landscaping. It has soft branches, beautiful tree posture, bright colors, and blooms twice a year. It solves the current situation of no flowers in summer and autumn in greening. shrub. Small-leaf lilac is an ornamental plant that can be enjoyed by both refined and popular tastes. It can be used as a potted plant in a study or hall, or as a cut flower in a vase. It is beautiful and pleasant. At the same time, clove has important medicinal value. The callus induction of Syringa microphylla is an important stage for plant regeneration. A higher callus induction rate lays a good foundation for the later callus differentiation. How to obtain a higher callus induction ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 梁艳安凤霞高美玲范震宇朱琨陈阳
Owner QIQIHAR UNIVERSITY
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